Skip to main content
SpringerLink
Log in

Myeloperoxidase Exocytosis from Activated Neutrophils in the Presence of Heparin

  • Published:
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

Exocytosis of myeloperoxidase (MPO) from activated neutrophils has been investigated in the presence of the anionic polysaccharide heparin. The optimal concentration of heparin (0.1 U/mL), which did not cause additional activation of cells (lack of augmentation of lysozyme exocytosis from specific and azurophilic granules), was determined. After preincubation of cells with heparin (0.1 U/mL) MPO exocytosis from neutrophils was stimulated by various activators (fMLP, PMA, plant lectins CABA and PHA-L) and was higher as compared to the effects of the activators alone. Experiments performed using MPO isolated from leukocytes have shown that heparin in the range of concentrations 0.1–50 U/mL had no effect on MPO peroxidase activity. Thus, the use of heparin at a concentration of 0.1 U/mL avoids the artifact caused by the “loss” of MPO due to its binding to neutrophils and increases the accuracy of the method of registration of degranulation of neutrophil azurophilic granules based on determination of the MPO concentration or its peroxidase activity in cell supernatants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

cyth b:

cytochalasin b

fMLP:

N-formyl-methionyl-leucyl-phenylalanine

MPO:

myeloperoxidase

o-DA:

o-dianisidine

PMA:

phorbol 12-myristate 13-acetate

PBS:

phosphate buffered saline

PAMPO:

MPO peroxidase activity

References

  1. Klebanoff, S.J., J. Leukoc. Biol., 2005, vol. 77, no. 5, pp. 598–625. doi 10.1189/jlb.1204697

    Article  CAS  PubMed  Google Scholar 

  2. Panasenko, O.M., Gorudko, I.V., and Sokolov, A.V., Biochemistry (Moscow), 2013, vol. 78, no. 13, pp. 1466–1489. doi 10.1134/S0006297913130075

    Article  CAS  Google Scholar 

  3. Lefkowitz, D.L., Mills, K., Morgan, D., and Lefkowitz, S.S., Proc. Soc. Exp. Biol. Med., 1992, vol. 199, no. 2, pp. 204–210. doi 10.3181/00379727-199-43348

    Article  CAS  PubMed  Google Scholar 

  4. Gorudko, I.V., Sokolov, A.V., Shamova, E.V., Grudinina, N.A., Drozd, E.S., Shishlo, L.M., Grigorieva, D.V., Bushuk, S.B., Bushuk, B.A., Chizhik, S.A., Cherenkevich, S.N., Vasilyev, V.B., and Panasenko, O.M., Biol. Open, 2013, vol. 2, no. 9, pp. 916–923. doi 10.1242/bio.20135314

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Lau, D., Mollnau, H., Eiserich, J.P., Freeman, B.A., Daiber, A., Gehling, U.M., Brümmer, J., Rudolph, V., Münzel, T., Heitzer, T., Meinertz, T., and Baldus, S., Proc. Natl. Acad. Sci. USA, 2005, vol. 102, no. 2, pp. 431–436. doi 10.1073/pnas.0405193102

    Article  CAS  PubMed  Google Scholar 

  6. Gorudko, I.V., Sokolov, A.V., Shamova, E.V., Grigorieva, D.V., Mironova, E.V., Kudryavtsev, I.V., Gusev, S.A., Gusev, A.A., Chekanov, A.V., Vasilyev, V.B., Cherenkevich, S.N., Panasenko, O.M., and Timoshenko, A.V., Arch. Biochem. Biophys., 2016, vol. 591, pp. 87–97. doi 10.1016/j.abb.2015.12.007

    Article  CAS  PubMed  Google Scholar 

  7. Van der Veen, B.S., de Winther, M.P., and Heeringa, P., Antioxid. Redox Signal., 2009, vol. 11, no. 11, pp. 2899–2937. doi 10.1089/ars.2009.2538

    Article  CAS  PubMed  Google Scholar 

  8. Gorudko, I.V., Cherkalina, O.S., Sokolov, A.V., Pulina, M.O., Zakharova, E.T., Vasilyev, V.B., Cherenkevich, S.N., and Panasenko, O.M., Russ. J. Bioorg. Chem., 2009, vol. 35, no. 5, pp. 566–575. doi 10.1134/S1068162009050057

    Article  CAS  Google Scholar 

  9. Panasenko, O.M., Mikhalchik, E.V., Gorudko, I.V., Grigorieva, D.V., Sokolov, A.V., Kostevich, V.A., Vasilyev, V.B., and Cherenkevich, S.N., Biophysics, 2016, vol. 61, no. 3, pp. 420–428. doi 10.1134/S0006350916030131.

    Article  CAS  Google Scholar 

  10. Franck, T., Minguet, G., Delporte, C., Derochette, S., Zouaoui Boudjeltia, K., Van Antwerpen, P., Gach, O., Deby-Dupont, G., Mouithys-Mickalad, A., and Serteyn, D., Free Radic. Res., 2015, vol. 49, no. 6, pp. 790–799. doi 10.3109/10715762.2015.1027197

    Article  CAS  PubMed  Google Scholar 

  11. Klinke, A., Nussbaum, C., Kubala, L., Friedrichs, K., Rudolph, T.K., Rudolh, V., Paust, H.J., Schröder, C., Benten, D., Lau, D., Szocs, K., Furtmüller, P.G., Heeringa, P., Sydow, K., Duchstein, H.J., Ehmke, H., Schumacher, U., Meinertz, T., Sperandio, M., and Baldus, S., Blood, 2011, vol. 117, no. 4, pp. 1350–1358. doi 10.1182/blood-2010-05-284513

    Article  CAS  PubMed  Google Scholar 

  12. Grigorieva, D.V., Gorudko, I.V., Sokolov, A.V., Kostevich, V.A., Vasilyev, V.B., Cherenkevich, S.N., and Panasenko, O.M., Bull. Exper. Biol. Med., 2016, vol. 161, no. 4, pp. 495–500. doi 10.1007/s10517-016-3446-7

    Article  CAS  Google Scholar 

  13. El Kebir, D., József, L., Pan, W., and Filep, J.G., Circ. Res., 2008, vol. 103, no. 4, pp. 352–359. doi 10.1161/01.RES.0000326772.76822.7a

    Article  CAS  PubMed  Google Scholar 

  14. Tyrrell, D.J., Horne, A.P., Holme, K.R., Preuss, J.M., and Page, C.P., Adv. Pharmacol., 1999, vol. 46, pp. 151–208. doi 10.1016/S1054-3589(08)60471-8

    Article  CAS  PubMed  Google Scholar 

  15. Boesman-Finkelstein, M., and Finkelstein, R.A., FEBS Letters, 1982, vol. 144, no. 1, pp. 1–5. doi 10.1016/0014-5793(82)80556-5

    Article  CAS  PubMed  Google Scholar 

  16. Sokolov, A.V., Ageeva, K.V., Kostevich, V.A., Berlov, M.N., Runova, O.L., Zakharova, E.T., and Vasilyev, V.B., Biochemistry (Moscow), 2010, vol. 75, no. 11, pp. 1361–1367. doi 10.1134/S0006297910110076

    Article  CAS  Google Scholar 

  17. Sokolov, A.V., Kostevich, V.A., Zakharova, E.T., Samygina, V.R., Panasenko, O.M., and Vasilyev, V.B., Free Radic. Res., 2015, vol. 46, no. 6, pp. 800–811. doi 10.3109/10715762.2015.1005615

    Article  CAS  Google Scholar 

  18. Baldus, S., Rudolph, V., Roiss, M., Ito, W.D., Rudolph, T.K., Eiserich, J.P., Sydow, K., Lau, D., Szöcs, K., Klinke, A., Kubala, L., Berglund, L., Schrepfer, S., Deuse, T., Haddad, M., Risius, T., Klemm, H., Reichenspurner, H.C., Meinertz, T., and Heitzer, T., Circulation, 2006, vol. 113, no. 15, pp. 1871–1878. doi 10.1161/CIRCULATIONAHA. 105.590083

    Article  CAS  PubMed  Google Scholar 

  19. Jog, N.R., Rane, M.J., Lominadze, G., Luerman, G.C., Ward, R.A., and McLeish, K.R., Am. J. Physiol. Cell Physiol., 2007, vol. 292, no. 5, pp. 1690–1700. doi 10.1152/ajpcell.00384.2006

    Article  CAS  Google Scholar 

  20. Videm, V., Scand. J. Immunol., 1996, vol. 43, no. 3, pp. 385–390. doi 10.1046/j.1365-3083.1996.d01-57.x

    Article  CAS  PubMed  Google Scholar 

  21. Léculier, C., Couprie, N., Adeleine, P., Leitienne, P., Francina, A., and Richard, M., Thromb. Res., 1993, vol. 69, no. 6, pp. 519–531. doi 10.1016/0049-3848(93)90056-T

    Article  PubMed  Google Scholar 

  22. Killeen, R., Wait, R., Begum, S., Gray, E., and Mulloy, B., Int. J. Exp. Pathol., 2004, vol. 85, no. 4, p. A69. doi 10.1111/j.0959-9673.2004.390af.x

    Article  PubMed Central  Google Scholar 

  23. Abdel-Latif, D., Steward, M., Macdonald, D.L., Francis, G.A., Dinauer, M.C., and Lacy, P., Blood, 2004, vol. 104, no. 3, pp. 832–839. doi 10.1182/blood-2003-07-2624

    Article  CAS  PubMed  Google Scholar 

  24. Timoshenko, A.V., Kayser, K., and Gabius, H.J., Methods Mol. Med., 1998, vol. 9, pp. 441–445. doi 10.1385/0-89603-393-1:441

    CAS  PubMed  Google Scholar 

  25. Brown, R.A., Lever, R., Jones, N.A., and Page, C.P., Br. J. Pharmacol., 2003, vol. 139, no. 4, pp. 845–853. doi 10.1038/sj.bjp.0705291

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Belarusian State University, Minsk, Belarus

    D. V. Grigorieva, I. V. Gorudko & S. N. Cherenkevich

  2. Institute for Experimental Medicine, St. Petersburg, Russia

    V. A. Kostevich, V. B. Vasilyev & A. V. Sokolov

  3. Federal Research and Clinical Center of Physical-Chemical Medicine, ul. Malaya Pirogovskaya 1a, Moscow, 119435, Russia

    V. A. Kostevich, O. M. Panasenko & A. V. Sokolov

  4. Saint-Petersburg University, St. Petersburg, Russia

    V. B. Vasilyev & A. V. Sokolov

  5. Pirogov Russian National Research Medical University, Moscow, Russia

    O. M. Panasenko

  6. Centre of Preclinical Translational Research, Almazov National Medical Research Centre, Saint-Petersburg, Russia

    A. V. Sokolov

Authors
  1. D. V. Grigorieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. V. Gorudko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. A. Kostevich
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. B. Vasilyev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. N. Cherenkevich
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. O. M. Panasenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. V. Sokolov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. M. Panasenko.

Additional information

Original Russian Text © D.V. Grigorieva, I.V. Gorudko, V.A. Kostevich, V.B. Vasilyev, S.N. Cherenkevich, O.M. Panasenko, A.V. Sokolov, 2018, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Grigorieva, D.V., Gorudko, I.V., Kostevich, V.A. et al. Myeloperoxidase Exocytosis from Activated Neutrophils in the Presence of Heparin. Biochem. Moscow Suppl. Ser. B 12, 136–142 (2018). https://doi.org/10.1134/S199075081802004X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S199075081802004X

Keywords

Search

Navigation