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Activation of mouse peritoneal macrophages by synthetic glyceroglycolipid liposomes

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Summary

Liposomes composed of chemically synthesized glyceroglycolipids, such as 1,2-dipalmityl-[β-cellobiosyl-(1′ → 3)]-glycerol (Cel-DAG), 1,2-dipalmityl-[β-lactosyl-(1′ → 3)]-glycerol, or 1,2-dipalmityl-[β-maltosyl-(1′ → 3)]-glycerol, were found to enhance protective immunity against transplantable tumor cells (sarcoma 180) in ICR mice. Peritoneal exudate cells prepared from mice treated in vivo with Cel-DAG showed cytostatic activity in vitro against the mouse leukemia cell line, EL-4. Adherent cells separated from this preparation showed similar activity. Peritoneal cells from polypeptone-injected mice acquired appreciable cytostatic activity when incubated in vitro in the presence of glyceroglycolipid liposomes. The adherent cell fraction alone showed rather weak cytostatic activity when pretreated with the glyceroglycolipids, and full activity was restored by supplementing with the nonadherent cell fraction. The ability of glycolipids to induce tumoricidal effects was affected by cholesterol content: with increasing cholesterol content, the activities decreased. Cholesterol-free glycolipid liposomes were taken more efficiently by macrophages than cholesterol-containing liposomes. Cholersterol modifies the surface property of glyceroglycolipid liposomes. Activation of macrophages is responsible for enhancement of protective immunity against tumor cells by injection of these glycolipids in vivo.

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Author notes

  1. Shoshichi Nojima

    Present address: Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko-machi, Tsukui-gun, 199-01, Kanagawa, Japan

Authors and Affiliations

  1. Department of Health Chemistry, Faculty of Pharmaceutical Sciences, the University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, 113, Tokyo

    Mikihiko Naito, Ichiro Kudo, Yukiko Mukai-Sato, Shoshichi Nojima & Keizo Inoue

  2. Central Research Division, Takeda Chemical Ind. Ltd., Jusohon-machi, Yodogawa-ku, Osaka, 532, Osaka, Japan

    Susumu Tsushima & Hiroaki Nomura

Authors
  1. Mikihiko Naito
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  2. Ichiro Kudo
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  3. Yukiko Mukai-Sato
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  4. Susumu Tsushima
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  5. Hiroaki Nomura
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  6. Shoshichi Nojima
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  7. Keizo Inoue
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Additional information

This work was supported in part by Grants-in-Aid (Nos. 58010010, and 59870076) for Scientific Research from the Ministry of Education, Science and Culture of Japan

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Naito, M., Kudo, I., Mukai-Sato, Y. et al. Activation of mouse peritoneal macrophages by synthetic glyceroglycolipid liposomes. Cancer Immunol Immunother 24, 158–164 (1987). https://doi.org/10.1007/BF00205594

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  • DOI: https://doi.org/10.1007/BF00205594

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