Abstract
Isolated rat liver cells have been exposed to 3 different lipid peroxidation-inducing agents, CCl4, FeCl3 and cumene hydroperoxide, and the rates of malonaldehyde production and of lipoprotein secretion have been compared. Results indicate that it is possible to induce a high degree of lipid peroxidation without inducing strong changes in lipoprotein secretion. Only in CCl4-poisoned hepatocytes is lipoprotein secretion strongly impaired. In this experimental condition, the effect of free radical scavengers, or inhibitors of lipid peroxidation, has been studied; the degree of covalent binding of CCl4 metabolites to hepatocyte proteins, as well as the behavior of both lipid peroxidation and lipoprotein secretion, have been evaluated. Promethazine and propyl gallate prevented malonaldehyde production, but neither agent reduced covalent binding nor improved secretion. Menadione, on the contrary, besides inhibiting malonaldehyde production, decreased covalent binding and protected against the impairment of secretion. These data lead to the conclusion that covalent binding of CCl4 metabolites, rather than lipid peroxidation products, accounts for the derangement of lipoprotein secretion in CCl4-poisoned liver cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Slater, T.F. (1972) Free Radical Mechanisms in Tissue Injury, (Lagnado, C., ed.), Pion Ltd., London.
Slater, T.F. (1978) in Biochemical Mechanisms of Liver Injury, (Slater, T.F., ed.), pp. 745–801, Academic Press, London.
Dianzani, M.U. (1978) in Biochemical Mechanisms of Liver Injury, (Slater, T.F., ed.), pp. 45–95, Academic Press, London.
Packer, J.L., Slater, T.F., and Willson, R.L. (1978) Life Sci. 23, 2617–2620.
Packer, J.L., Willson, R.L., Bahnemann, D., and Asmus, K.D. (1980) J. Chem. Soc. Perkin Trans. II, 296–299.
Recknagel, R.O. (1967) Pharmacol. Rev. 19, 145–208.
Dianzani, M.U., Gabriel, L., Gravela, E., and Paradisi, L. (1976) in Recent Advances in Biochemical Pathology: Toxic Liver Injury, pp. 29–38, Minerva Medica, Torino.
Dianzani, M.U., Bertone, G., Bonelli, G., Canuto, R.A., Feo, F., Gabriel, L., Gravela, E., and Pernigotti, L. (1976) Med. Biol. Environ. 4, 345–365.
Weddle, C.C., Hornbrook, K.R., and McCay, P.B. (1976) J. Biol. Chem. 251, 4973–4978.
Poli, G., Poli Chiono, M., Slater, T.F., Dianzani, M.U., and Gravela, E. (1978) Biochem. Soc. Trans. 6, 589–591.
Poli, G., Gravela, E., Albano, E., and Dianzani, M.U. (1979) Exp. Mol. Pathol. 30, 116–127.
Gravela, E., Albano, E., Dianzani, M.U., Poli, G., and Slater, T.F. (1979) Biochem. J. 178, 509–512.
Hochstein, P., Nordenbrand, K., and Ernster, L. (1964) Biochem. Biophys. Res. Commun. 14, 323–328.
O'Brien, P.J., and Rahimtula, A. (1975) J. Agric. Food Chem. 23, 154–158.
Viña, J., Hems, R., and Krebs, H.A. (1978) Biochem. J. 170, 627–630.
Gravela, E., Poli, G., Albano, E., and Dianzani, M.U. (1977) Exp. Mol. Pathol. 27, 339–352.
Bernheim, F., Bernheim, M.L.C., and Wilbur, K.M. (1948) J. Biol. Chem. 174, 257–264.
Rao, K.S., and Recknagel, R.O. (1969) Exp. Mol. Pathol. 10, 219–228.
Iyanagi, T., and Yamazaki, I., (1969) Biochim. Biophys. Acta 172, 370–381.
Wills, E.D. (1972) Biochem. Pharmacol. 21, 1879–1883.
Rees, K.S., Sinha, K.P., and Spector, W.G. (1961) J. Path. Bact. 81, 107–118.
Cheeseman, K., and Slater, T.F. (1981) Proc. Int. Symposium on Lipid Peroxidation, Uxbridge, September 1979, Brunel University Press, in press.
May, H.E., and McCay, P.B. (1968) J. Biol. Chem. 243, 2288–2295.
Pfeifer, P.M., and McCay, P.B. (1972) J. Biol. Chem. 247, 6763–6769.
Fong, K.L., McCay, P.B., and Poyer, J.L. (1973) J. Biol. Chem. 248, 7792–7797.
Högberg, J., Moldéus, P., Arborgh, B., O'Brien, P.J., and Orrenius, S. (1975) Eur. J. Biochem. 59, 457–462.
Poli, G., Gravela, E., Chiarpotto E., Albano, E., and Dianzani, M.U. (1979) IRCS Med. Sci. 7, 558.
Svingen, B.A., O'Neal, F.O., and Aust, S.D. (1978) Photochem. Photobiol. 28, 803–809.
Hrycay, E.G., Gustafsson, J.A., Ingelman-Sundberg, M., and Ernster, L. (1976) Eur. J. Biochem. 61, 43–52.
De Toranzo, E.G.D., Diaz Gomez, M.I., and Castro, J.A. (1975) Biochem. Biophys. Res. Commun. 64, 823–828.
Gillette, J.R., Mitchell, J.R., and Brodie, B.B. (1974) Annu. Rev. Pharmacol. 14, 271–288.
Gravela, E. (1975) Res. Commun. Chem. Pathol. Pharmacol. 11, 615–625.
Author information
Authors and Affiliations
About this article
Cite this article
Dianzani, M.U., Poli, G., Gravela, E. et al. Influence of lipid peroxidation on lipoprotein secretion by isolated hepatocytes. Lipids 16, 823–829 (1981). https://doi.org/10.1007/BF02535037
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02535037