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Effect of buffer constituents on rat liver 3-hydroxy-3-methyl glutaryl coenzyme A reductase

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Lipids

Abstract

Rat liver microsomes prepared in Tris buffer exhibited 3 to 10 times higher 3-hydroxy-3-methyl glutaryl CoA reductase specific activity than microsomes prepared with potassium phosphate buffer. This higher activity was observed in rats killed during mid-light cycle, but microsomes from rats killed during mid-dark cycle showed no significant difference in enzyme activity between buffers. When microsomes prepared in the 2 different buffers were preincubated with ATP and MG++, enzyme activity was inhibited to the same extent. The cytosol fraction in each of the 2 different buffer preparations possessed similar phosphatase activity. The higher 3-hydroxy-3-methyl reductase activity in Tris buffer, therefore, does not appear to be due to differences in phosphorylation or dephosphorylation activity.

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Authors and Affiliations

  1. Departments of Pathology and Medicine, Harbor-UCLA Medical Center, 1000 West Carson St., 90509, Torrance, CA

    M. V. Srikantaiah, N. Noble, L. Orenstein & R. J. Morin

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  1. M. V. Srikantaiah
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  2. N. Noble
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  3. L. Orenstein
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  4. R. J. Morin
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Srikantaiah, M.V., Noble, N., Orenstein, L. et al. Effect of buffer constituents on rat liver 3-hydroxy-3-methyl glutaryl coenzyme A reductase. Lipids 16, 934–936 (1981). https://doi.org/10.1007/BF02535001

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  • DOI: https://doi.org/10.1007/BF02535001

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