Abstract
1-0-Hexadecyl-2-0-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) at 10−10-10−9 M induced slow contraction of isolated guinea-pig ilcal muscles and the contraction persisted for a long time. At a higher concentration of 10−7 M, this phospholipid induced more rapid, but not greater, contraction. At higher concentrations (10−6-10−5 M), this phospholipid induced a biphasic response: rapid contraction followed by relaxation. At high concentrations, this compound inhibited acetylcholine-induced contractions. The stimulatory effect of this phospholipid was ca. 300 times that of 1-palmitoyl-2-0-acetyl-sn-glycero-3-phosphocholine, while its inhibitory potency on induced contraction was similar to those of 1-palmitoyl-2-0-acetyl-sn-glycero-3-phosphocholine and its lyso derivative. It was suggested that the differences in effects on contraction of different concentrations of 1-0-hexadecyl- and 1-palmitoyl-2-0-acetyl-sn-glycero-3-phosphocholine were due to the dual effects of these compounds on the ileum: a strong stimulatory effect and a moderate inhibitory effect on contraction.
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Tokumura, A., Harada, K., Fukuzawa, K. et al. Biphasic action of platelet-activating factor on isolated guinea-pig ileum. Lipids 18, 848–850 (1983). https://doi.org/10.1007/BF02534647
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DOI: https://doi.org/10.1007/BF02534647