Tripeptide glycyl-prolyl-proline (PGP), a regulatory peptide of the glyproline family, possesses a pronounced anti-inflammatory effect primarily due to its ability to prevent secretion of the proinflammatory mediator histamine by rat peritoneal mast cells. Activation of mast cell with synacthen (ACTH1-24) and substance 48/80 leads to an increase in intracellular calcium concentration. Pretreatment of mast cells with PGP prevented calcium entry into the cytoplasm from both intercellular space and intracellular stores. Acetylated peptide (N-AcPGP) produced a similar effect on histamine release and intracellular calcium content in mast cells activated with synacthen. These findings indicate that both forms of the peptide can stabilize mast cells and prevent intracellular calcium increase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Haddox JL, Pfister RR, Muccio DD, Villain M, Sommers CI, Chaddha M, Anantharamaiah GM, Brouillette WJ, DeLucas LJ. Bioactivity of peptide analogs of the neutrophil chemoattractant, N-acetyl-proline-glycine-proline. Invest. Ophthalmol. Vis. Sci. 1999:40(10):2427-2429.
Kim SD, Lee HY, Shim JW, Kim HJ, Yoo YH, Park JS, Baek SH, Zabel BA, Bae YS. Activation of CXCR2 by extracellular matrix degradation product acetylated Pro-Gly-Pro has therapeutic effects against sepsis. Am. J. Respir. Crit. Care Med. 2011:184(2):243-251.
Pfister RR, Haddox JL, Sommers CI, Lam KW. Identification and synthesis of chemotactic tripeptides from alkali-degraded whole cornea. A study of N-acetyl-proline-glycine-proline and N-methyl-proline-glycine-proline. Invest. Ophthalmol. Vis. Sci. 1995:36(7):1306-1316.
Samonina G, Ashmarin I, Lyapina L. Glyproline peptide family: review on bioactivity and possible origins. Pathophysiology. 2002:8(4):229-234.
Suzuki Y, Inoue T, Ra C. Calcium signaling in mast cells: focusing on L-type calcium channels. Adv. Exp. Med. Biol. 2012:740):955-977. doi: 10.1007/978-94-007-2888-2_44.
Tomita U, Inanobe A, Kobayashi I, Takahashi K, Ui M, Katada T. Direct interactions of mastoparan and compound 48/80 with GTP-binding proteins. J. Biochem. 1991:109(1):184-189.
Umarova BA, Bondarenko NS, Kopylova GN, Samonina GE. The effect of PGP on β-hexosaminidase and histamine secretion in rat peritoneal mast cells in vitro. Biochem. (Mosc). Suppl. Ser. A. Membr. Cell Biol. 2011:5(3):237-241.
V’unova TV, Andreeva LA, Shevchenko KV, Shevchenko VP, Bobrov MY, Bezuglov VV, Myasoedov NF. Binding of tripeptide Pro-Gly-Pro labeled at the C-terminal proline residue to plasma membranes of the rat forebrain. Dokl. Biol. Sci. 2008:419:95-96.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 4, pp. 475-478, April, 2016
Rights and permissions
About this article
Cite this article
Bondarenko, N.S., Kurenkova, A.D., Nikishin, D.A. et al. Effect of Prolyl-Glycyl-Proline (PGP) and Its Acetylated Form (N-AcPGP) on Calcium Level in the Cytoplasm of Rat Peritoneal Mast Cells. Bull Exp Biol Med 161, 487–490 (2016). https://doi.org/10.1007/s10517-016-3444-9
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3444-9