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Analytical13C NMR: Detection, quantitation, and positional analysis of butyrate in butter oil

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Lipids

Abstract

The amount of butyrate contained in a complex mixture of butter oil triglycerides was 10.3 mole % as determined by natural abundance13C Fourier transform pulse nuclear magnetic resonance (NMR) spectroscopy. This NMR technique also demonstrated the primary isomeric positioning (>97%) of the butyrl group without the need for altering or fractionating the fat mixture.

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Authors and Affiliations

  1. Dairy Laboratory, Eastern Regional Research Center ARS, USDA, 19118, Philadelphia, PA

    P. E. Pfeffer, Joseph Sampugna (Senior Sabbatical Fellow supported in part by DRINC and University of Maryland, College Park, MD) & Daniel P. Schwartz

  2. Varian Associates, 94303, Palo Alto, CA

    James N. Shoolery

Authors
  1. P. E. Pfeffer
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  2. Joseph Sampugna
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  3. Daniel P. Schwartz
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  4. James N. Shoolery
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Pfeffer, P.E., Sampugna, J., Schwartz, D.P. et al. Analytical13C NMR: Detection, quantitation, and positional analysis of butyrate in butter oil. Lipids 12, 869–871 (1977). https://doi.org/10.1007/BF02533278

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  • DOI: https://doi.org/10.1007/BF02533278

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