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Sensitive fluorometric method for tissue tocopherol analysis

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Lipids

Abstract

A sensitive, highly reproducible method for tissue tocopherol analysis that combines saponification in the presence of large amounts of ascorbic acid to remove interfering substances, extraction of the nonsaponifiable lipids with hexane, and fluorometric measurement of the tocopherol is presented. The nonsaponifiable lipid phase contained only one fluorochrome in the 290 nm excitation and 330 nm emission range, and it was identified as tocopherol by thin layer and column chromatography. Column chromatography of the hexane extract of a saponified,14C-tocopherolspiked microsomal fraction showed that no measurable oxidation to tocopherylquinone has occurred. The fluorometric method for tocopherol analysis was applied to homogenates and subcellular fractions from rat liver, kidney, lung, and heart and red blood cells. The heavy mitochondrial and microsomal fractions had the highest subcellular concentrations of tocopherol.

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Author information

Author notes

  1. Steve L. Taylor

    Present address: Food Hygiene Division, Dept. of Nutrition, Letterman Army Institute for Research, Presidio, 94129, San Francisco, CA

Authors and Affiliations

  1. Department of Food Science and Technology, University of California, 95616, Davis, California

    Steve L. Taylor

  2. Department of Biochemistry, College of Medicine, University of Vermont, 05401, Burlington, Vermont

    Merton P. Lamden

  3. Department of Food Science and Technology, University of California, 95616, Davis, California

    AL. L. Tappel

Authors
  1. Steve L. Taylor
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  2. Merton P. Lamden
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  3. AL. L. Tappel
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Taylor, S.L., Lamden, M.P. & Tappel, A.L. Sensitive fluorometric method for tissue tocopherol analysis. Lipids 11, 530–538 (1976). https://doi.org/10.1007/BF02532898

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  • DOI: https://doi.org/10.1007/BF02532898

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