Skip to main content
SpringerLink
Log in

The Use of Kits in the Analysis of Tissue Lipids Requires Validation

  • Methods
  • Published:
Lipids

Abstract

The ready availability and ease of use of kits for the measurement of serum lipids has greatly facilitated these measurements. In many cases it would be convenient to use these kits in the determination of lipid concentrations in tissues. The successful application of serum kits in tissue analysis requires that two important issues be considered. First, the solvent system for the extraction of the lipids and the solvent used for analysis by the kit must be compatible with the reactions in the kit. Second, the concentration range in the analyzed solution must be within the range for which the kit is used. We report here that lipids in liver and adipose tissues may be significantly underestimated by the use of some kits. We recommend that the use of kits for tissue analysis of lipids be validated for the specific analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Abbreviations

NIH:

National Institutes of Health

NIDDK:

National Institute of Diabetes and Digestion and Kidney Disease

References

  1. Zhu Y, Jong MC, Frazer KA, Gong E, Krauss RM, Cheng JF, Boffelli D, Rubin EM (2000) Genomic interval engineering of mice identifies a novel modulator of triglyceride production. Proc Natl Acad Sci USA 97(3):1137–1142

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Bastida S, Garcia-Linares MC, Viejo J, Garcia-Arias MT, Sanchez-Muniz FJ (2006) Effect of olive oil-fried sardine consumption on cholesterol content in the serum, lipoproteins, spleen and adipose tissue of hypercholesterolemic rats. Ann Nutr Metab 50(1):54–58

    Article  CAS  PubMed  Google Scholar 

  3. Hirako S, Kim HJ, Arai T, Chiba H, Matsumoto A (2010) Effect of concomitantly used fish oil and cholesterol on lipid metabolism. J Nutr Biochem 21(7):573–579

    Article  CAS  PubMed  Google Scholar 

  4. Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH (2007) Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice. J Nutr 137(7):1776–1782

    CAS  PubMed  Google Scholar 

  5. Zlatkis A, Zak B (1969) Study of a new cholesterol reagent. Anal Biochem 29(1):143–148

    Article  CAS  PubMed  Google Scholar 

  6. Folch J, Lees M, Sloane Stlanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226(1):497–509

    CAS  PubMed  Google Scholar 

  7. Biggs HG, Erikson JM, Moorehead WR (1975) A manual colorimetric assay of triglycerides in serum. Clin Chem 21(3):437–441

    CAS  PubMed  Google Scholar 

  8. Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234(3):466–468

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

Funding for the work described in this paper was provided by NIH/NIDDK Funded U24 DK059630 (University of Cincinnati Mouse Metabolic Phenotyping Center) and NIH R01 HL055362.

Author information

Authors and Affiliations

  1. Department of Cancer Biology, University of Cincinnati, Cincinnati, USA

    T. Rider

  2. Department of Medicine, University of Washington, Seattle, USA

    R. C. LeBoeuf

  3. Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, USA

    Patrick Tso & R. J. Jandacek

Authors
  1. T. Rider
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. C. LeBoeuf
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Patrick Tso
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. J. Jandacek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Patrick Tso.

Ethics declarations

Conflict of interest

The authors have no conflicts of interest.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rider, T., LeBoeuf, R.C., Tso, P. et al. The Use of Kits in the Analysis of Tissue Lipids Requires Validation. Lipids 51, 497–504 (2016). https://doi.org/10.1007/s11745-016-4134-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11745-016-4134-0

Keywords

Search

Navigation