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Enzymatic Quantification of Liver Lipids After Folch Extraction

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Kupffer Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2164))

Abstract

The determination of the lipid content in liver offers the potential to investigate metabolic alterations in different research contexts. Here, we describe a method to determine cholesterol, triglycerides, and phospholipids in liver samples based on total lipid isolation by a 2:1 chloroform–methanol mixture (Folch extraction) and specific enzymatic colorimetric microassays in plate.

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References

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Author information

Authors and Affiliations

  1. Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Mirko E. Minniti, Osman Ahmed & Matteo Pedrelli

Authors
  1. Mirko E. Minniti
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  2. Osman Ahmed
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  3. Matteo Pedrelli
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Corresponding authors

Correspondence to Mirko E. Minniti or Matteo Pedrelli .

Editor information

Editors and Affiliations

  1. Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    Myriam Aouadi

  2. Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    Valerio Azzimato

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Minniti, M.E., Ahmed, O., Pedrelli, M. (2020). Enzymatic Quantification of Liver Lipids After Folch Extraction. In: Aouadi, M., Azzimato, V. (eds) Kupffer Cells. Methods in Molecular Biology, vol 2164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0704-6_11

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  • DOI: https://doi.org/10.1007/978-1-0716-0704-6_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0703-9

  • Online ISBN: 978-1-0716-0704-6

  • eBook Packages: Springer Protocols

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