Summary
Based on a stoichiometric displacement model for retention (SDM-R) in liquid chromatography, the two linear plots, log/(a constant relating to the affinity of one mole of solute to the stationary phase) andZ (the total moles of the displacing agent released at the interface between stationary phase and solute molecules as one mole of the solute is absorbed by the stationary phase) of small solutes and biopolymers versus the reciprocal of absolute temperature, l/T, in reversed-phase liquid chromatography were theoretically derived and experimentally proved to be linear and to have a common point called the temperature convergent point. The two linear plots could be used instead of the plot of logk to l/T which is normally only valid for small solutes but not for biopolymers for investigations of chromatographic thermodynamics and temperature convergence. The average convergence temperature (T conv) of biopolymers was theoretically derived to equal the ratio between the slope and the intercept from either one of the two linear plots and to be 130.8±9.3°C for five proteins which are very close to those of biopolymers by calorimetry. A new methodology to investigate chromatographic thermodynamics and possibly for investigating the temperature convergence of biopolymers in the process of protein folding is also presented.
Similar content being viewed by others
References
Makhatadze, G.I.; Privalov, P.L.J. Mol. Biol 1993 232, 639–659.
Privalov, P.L.Adv. Protein Chem 1979,33, 167–241.
Berendsen, G.E.; Galan, L.J. Chromatogr 1980,196, 21–37.
Dufek, P.J. Chromatogr 1983,281, 49–58.
Bidlingmeyer, B.A.; Deming, S.N.; Price, E.P.; et al.J. Chromatogr 1979,186, 419–434.
Colin, H.; Krstulovic, A.M.; Gonnord, M.F.; et al.Chromatographia 1983,17, 9–13.
Geng, X.D.; Regnier, F.E.Chromatographia 1994,38, 158–162.
Bondi, A.J. Phys. Chem 1964,68, 441–451.
Rekker, R.F.The Hydrophobic Fragment Constant, Elsever Scientific Publishing Co., Amsterdam,1977, p. 350.
Zhang, J.; Ma, Z.K.; Geng, X.D.Acta Chimica Sinica 1999,57, 967–973.
Zhang, J.; Wang, Y.; Geng, X.D.J. Anal. Sci 2000,16, 5–9.
Bai, Q.; Zhang, R.Y.; Geng, X.D.Acta Chimica Sinica 1997,55, 1025–1029.
Bai, Q.; Geng, X.D.Sepu (Chinese) 2000,18, 189–193.
Geng, X.D.; Regnier, F.E.J. Chromatogr 1985,332, 147–168.
Geng, X.D.; Regnier, F.E.J. Chromatogr 1984,296, 15–30.
Geng, X.D.Science in China (B) 1995,25, 264–371.
Chang, H.; Guo, L.; Feng, W.; Geng, X.D.Chromatographia 1992,34, 589–596.
Geng, X.D.Acta Chimica Sinica 1995,53, 369–375.
Geng, X.D.Atca Chimica Sinica 1996,54, 497–503.
Shi, Y.L.; Ma, F.; Geng, X.D.Chem. J. Chin. Univ 1994,15, 1288–1291.
Baldwin, R.L.; Muller, N.Proc. Natl. Acad. Sci. USA 1992,89 7110–7113.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bai, Q., Geng, X. The temperature convergence of biopolymers in reversed-phase liquid chromatography. Chromatographia 54, 335–338 (2001). https://doi.org/10.1007/BF02492679
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02492679