Summary
The similarities and differences in retention characteristics of ferrocene-bonded and other three silica-based phases have been elucidated by the use of linear solvation energy relation-ships (LESRs). These phases were investigated in a common mobile phase. The results of LSERs on ferrocene-bonded phases are quite different from other silica-based phases. Retention on all other silica-based phases is dominated by two factors: the solute size and hydrogen bond acceptor basicity. While on ferrocene-bonded silica phase, the retention is dominated by excess molar refraction and hydrogen bond acceptor basicity. The s coefficient is small but positive which is unlike the common reversed phase stationary phases. Ther coefficient is much larger and positive showed the new phase has stronger π-π interaction to solutes than all other phases, and high selectivity to polyaromotic hydrocarbons.
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Tan, L.C.; Carr, P.W.; Abraham, M.H.J. Chromatogr. A 1996,752, 1–18.
Tan, L.C.; Carr, P.W.J. Chromatogr. A 1998,799, 1–19.
Li, J.; Carr, P.W.Anal. Chim. Acta. 1996,334, 239–250.
Abraham, M.H.; Chadha, H.S.; Leitao, R.A.E.; Mitehell, R.C.; Lambert, W.J.; Kaliszan, R.; Nasal, A.; Haber, P.J. Chromatogr. A 1997,766, 35–47.
Zhao, J.H.; Carr, P.W.Anal. Chem. 1998,30, 3619–3628.
Kamlet, M.J.; Taft, R.W.J. Am. Chem. Soc. 1976,98, 377–383.
Kamlet, M.J.; Taft, R.W.J. Am. Chem. Soc. 1976,98, 2886–2894.
Mc Gowan, J.C.J. Chem. Technol. Biotechnol. 1984,34 A, 38–42.
Abraham, M.H.; Mc Gowan, J.C.Chromatographia 1987,23, 243–253.
Abraham, M.H.Chem. Soc. Rev. 1993,22, 73.
Huai, Q.Y.; Wang, X.L.; Zuo, Y.M.Chromatographia 2002,55, 549.
Carr, P.W.; Li, J.; Dallas, A.J.; Eikens, D.I.; Tan, L.C.J. Chromatogr. A 1993,656, 113–133.
Abraham, M.H.; Whiting, G.S.; Fuchs, R.; Chambers, E.J.J. Chem. Soc. Perkin Trans. 1990,2, 291–300.
Eikens, D.K.Ph.D. University of Minnesota, Minneapolis,1993.
Horvath, C.; Melander, W.; Molnar, I.J. Chromatogr. 1976,125, 129 156u7.
Hildebrand, J.H.; Prausnitz, J.M.Regular and Related Solutions; Van Norstrand; New York1970.
Riddick, J.A.; Bunger, W.B.; Sakano, T.K.Organic Solvents, 4th Ed.; Wlley-Interscience; New York,1986.
Park, J.H.; Carr P.W.J. Chromatogr. 1989,465, 123–136.
Brodelanne, O.; Deville, M.H.; Félix, G.; Labrugère, C.; Thienpont, A.; Vidal, C.Chromatographia 2000,52, 51–55.
Dorsey, J.G.; Dill, K.A.Chem. Rev. 1989,89, 331–346.
Martire, D.E.; Boehm, R.E.J. Phys. Chem. 1983,87, 1045–1062.
Scott, R.P.W.; Simpson, C.F.Frarday Symp. Chem. Soc. 1980,15, 69–73.
Snyder, L.R.Principles of Adsorption Chromatography, Marcel Dekker: New York,1968.
Melander, w.R.; Horvath, C. InHigh Performance Liquid Chromatography, Advances and Perspectives; Horvath, C., Ed.; Academic Press; New York,1980,Vol. 2., 113.
Jaroniec, M.; Martire, D.E.; Borowko, M.Adv. Colloid Interface Sci. 1985,22, 177–227.
Everett, D.H. InAdsorption from Solution; Ottewill, R.H.; Rochester, C.H.; Smith, A.L., Eds. Academic Press: New York,1983.
Hammers, W.E.; Meurs, G.J.; DeLingny, C.I.J. Chromatogr. 1982,246, 169–189
Tan, L.Ph.D., University of Minnesota, Minneapolis,1994.
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Huai, Q.Y., You, J.M., Wang, Q. et al. Evaluation of the retention characteristics of ferrocene-bonded silica stationary phase and other silica-based reversed phases for HPLC using linear solvation energy relationships. Chromatographia 57, 709–713 (2003). https://doi.org/10.1007/BF02491755
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DOI: https://doi.org/10.1007/BF02491755