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Determination of cortisol and cortisone in human morning and overnight urine by thin-layer chromatography and fluorescence derivatisation with isonicotinic acid hydrazide

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  • Thin-Layer Chromatography
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Summary

A routine method for the measurement of cortisol and cortisone in human morning and overnight unine has been developed. The method includes liquid-liquid extraction, alkaline wash of the solvent, thin-layer chromatography (TLC), formation of isonicotilyl hydrazones of Δ4-3-keto-corticosteroids and their quantification by fluorescence densitometry. Cortisol excretion (mean values,n=22) was 3918 ng h−1 (07.00–08.00), 1743 ng h−1 (08.00–09.00) and 24300 ng night−1; corresponding cortisone values were 2356 ng h−1, 2144 ng h−1 and 22780 ng night−1. The advantage of the method is its sensitivity, being comparable with that of HPLC methods, its specificity (fluorescence of isonicotinyl hydrazones) and simplicity of separation (TLC) and quantification (densitometry).

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  1. Department of Animal Physiology, University of Bayreuth, 95440, Bayreuth, Germany

    M. Fenske

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  1. M. Fenske
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Fenske, M. Determination of cortisol and cortisone in human morning and overnight urine by thin-layer chromatography and fluorescence derivatisation with isonicotinic acid hydrazide. Chromatographia 52, 810–814 (2000). https://doi.org/10.1007/BF02491009

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  • DOI: https://doi.org/10.1007/BF02491009

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