Abstract
Oat coleoptile segments were treated with or without 10 mM galactose in the presence or absence of 10 μM IAA and various concentrations of mannitol (pre-incubation). Auxin-induced growth was inhibited by galactose. Segments were then transferred to buffer solutions containing or not containing 10 mM galactose (post-incubation). Expansion growth due to rapid water absorption was observed. The expansion growth during the post-incubation was inhibited by galactose when galactose was applied during the post-incubation period or all through the pre- and post-incubation but was not affected by galactose when it was applied only during the pre-incubation. This result indicates that the galactose effect on the expansion growth is due to its inhibitory action during the post-incubation period. Galactose has been reported to be a specific inhibitor for cell wall synthesis. Thus, it is suggested that the expansion growth during post-incubation requires cell wall synthesis and is not just the process of passive water absorption. The primary action of auxin does not seem to require new synthesis of polysaccharides.
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References
Baker, D.B. andP.M. Ray. 1965. Relation between effects of auxin on cell wall synthesis and cell elongation. Plant Physiol.40: 360–368.
Cleland, R. 1971. Cell wall extension. Ann. Rev. Plant Physiol.22: 197–222.
—. 1956. The residual effect of auxin on the cell wall. Plant Physiol31: 350–354.
Inouhe, M., R. Yamamoto andY. Masuda. 1984. Auxin-induced changes in the molecular weight distribution of cell wall xyloglucan inAvena coleoptiles. Plant Cell Physiol.25: 1341–1351.
—,—and—. 1986. Inhibition of auxin-induced cell elongation inAvena coleoptile segments by galactose: Its effect on UDP-glucose formation. Physiol. Plant.66: 370–376.
Kazama, H. andM. Katsumi. 1976. Biphasic response of cucumber hypocotyl sections to auxin. Plant Cell Physiol.17: 467–473.
Labavitch, J.M. andP.M. Pay. 1974. Relationship between promotion of xyloglucan metabolism and induction of elongation by indoleacetic acid. Plant Physiol.54: 499–502.
Masuda, Y. 1978. Auxin-induced cell wall loosening. Bot. Mag. Tokyo Special Issue1: 103–123.
—. 1984. Cell-wall changes during auxin-induced cell extension. Mechanical properties and constituent polysaccharides of the cell wall.In: C.T. Brett and J.R. Hillman, ed., Biochemistry of Plant Cell Walls p. 269–300. Cambridge Univ. Press, Cambridge.
Nishitani, K. andY. Masuda. 1981. Auxin-induced changes in the cell wall structure: Changes in the sugar compositions, intrinsic viscosity and molecular weight distribution of matrix polysaccharides of the epicotyl cell wall ofVigna angularis. Physiol. Plant.52: 482–494.
Ordin, L. andJ. Bonner. 1957. Effect of galactose on growth and metabolism ofAvena coleoptile sections. Plant. Physiol.32: 212–215.
Penny, P., D. Penny, D. Marshall andJ.K. Heyes. 1972. Early responses of excised stem segments to auxin. J. Exp. Bot.23: 23–36.
Ray, P.M. 1962. Cell wall synthesis and cell elongation in oat coleoptile tissue. Amer. J. Bot.49: 928–939.
Sakurai, N. andY. Masuda. 1977. Effect of indole-3-acetic acid on cell wall loosening: Changes in mechanical properties and noncellulosic glucose content ofAvena coleoptile cell wall. Plant Cell Physiol.18: 587–594.
Vanderhoef, L.N., C.A. Stahl, C.A. Williams, K.A. Brinksman andJ.G. Greenfield. 1978. Additional evidence for separate responses to auxin in soybean hypocotyl. Plant Physiol.57: 817–819.
Yamamoto, R., N. Sakurai andY. Masuda. 1980. Effect of auxin on the structure of hemicelluloses ofAvena coleoptiles. Plant Cell Physiol.21: 373–381.
—,—and—. 1981. Inhibition of auxin-induced cell elongation by galactose. Physiol. Plant.53: 543–547.
—. 1984. Galactose inhibition of auxin-induced cell elongation in oat coleoptile segments. Physiol. Plant.61: 321–326.
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Yamamoto, R. Effect of galactose on auxin-induced cell elongation in oat coleoptile segments in mannitol solutions. Bot. Mag. Tokyo 100, 43–49 (1987). https://doi.org/10.1007/BF02488419
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DOI: https://doi.org/10.1007/BF02488419