Abstract
We examined the expression of DNA topoisomerase IIα (Topo IIα) immunohistochemically using a monoclonal antibody and compared its proliferative potential [MIB-1 labeling index (LI)] and recurrence to verify the possible influence of Topo IIα on the progress of meningiomas. The reverse transcription-polymerase chain reaction (RT-PCR) assay was also performed to evaluate the expression of Topo IIα mRNA. Formalin-fixed, paraffin-embedded tissue sections of 52 meningiomas (18 meningothelial types, 16 fibrous types, 4 transitional types, 4 psammomatous types, 1 angiomatous type, 1 secretory type, 5 atypical types, and 3 anaplastic types) were used for immunostaining. The Topo IIα labeling index (LI) was 1.4±1.9% (mean ± SE) in benign meningiomas and 4.5±1.6% in atypical or anaplastic meningiomas, representing significant differences between them (P<0.0001). RT-PCR assay revealed that Topo IIα mRNA expression was associated with Topo IIα LI. A significant correlation was seen between Topo IIα LI and MIB-1 LI (r=0.517;P<0.01). Recurrence was significantly more frequent in patients with more than 1.5% of Topo IIα LI than in those with 1.5% or less (P<0.005). In conclusion, Topo IIα protein and mRNA expression correlated with clinical malignancy and the potential for predicting the regrowth of meningiomas.
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Kunishio, K., Morisaki, K., Matsumoto, Y. et al. DNA topoisomerase IIα protein and mRNA expression in intracranial meningiomas. Brain Tumor Pathol 17, 105–110 (2000). https://doi.org/10.1007/BF02484281
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DOI: https://doi.org/10.1007/BF02484281