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Expression of DNA topoisomerase I, IIα, and IIβ in human brain tumors

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Abstract

We investigated the expression of DNA topoisomerase I (Topo I), IIα (Topo IIα) and IIβ (Topo IIβ) mRNA using reverse transcription-polymerase chain reaction (RT-PCR) assay in 31 human brain tumors, and examined the relationship between DNA topoisomerase mRNA expression and Topo IIα and MIB-1 positive index (PI) as a cell proliferation marker. Topo IIα mRNA was expressed in 11 of 31 cases, and Topo I and IIβ were each expressed in 18 of 31 cases. A significant correlation was seen between the MIB-1 PI and Topo IIα PI (P<0.001). The cases with overexpression of Topo IIα mRNA had significantly high MIB-1 and Topo IIα PI (P<0.0001). There was no significant correlation between Topo I and IIβ mRNA expression and MIB-1 PI. We concluded that it was useful as a cell proliferation marker to analyze the expression of Topo IIα mRNA using RT-PCR in human brain tumors.

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Authors and Affiliations

  1. Department of Neurological Surgery, Kagawa Medical University, 1750-1 Ikenobe, Mikicho, 761-0701, Kitagun, Kagawa, Japan

    Kuniaki Morisaki, Setsuko Kuroda, Yoshihito Matsumoto, Katsuzo Kunishio & Seigo Nagao

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  1. Kuniaki Morisaki
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  2. Setsuko Kuroda
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  3. Yoshihito Matsumoto
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  4. Katsuzo Kunishio
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  5. Seigo Nagao
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Morisaki, K., Kuroda, S., Matsumoto, Y. et al. Expression of DNA topoisomerase I, IIα, and IIβ in human brain tumors. Brain Tumor Pathol 17, 7–13 (2000). https://doi.org/10.1007/BF02478912

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  • DOI: https://doi.org/10.1007/BF02478912

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