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ChickenMhc alloantiserum cross-reactivity analysis by hemagglutination and flow cytometry

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Abstract

The major histocompatibility complex (Mhc) haplotype in the chicken is generally determined by the use of alloantisera in a hemagglutination assay. This method restricts haplotype determination to antigens expressed on the surface of erythrocytes which includes class I (B-F) and class IV (B-G) antigens as well as any other polymorphic molecules on these cells. Alloantisera can result in complex cross-reactivity patterns. We describe here the analysis of 53 alloantisera made withinMhc-congenic lines. Each antiserum was tested by hemagglutination with erythrocytes and by flow cytometry with erythrocytes and peripheral white blood cells of sevenMhc haplotypes;B 2, B5, B12, B15, B19, andB 21. Five types of antiserum were identified based on their reactivity to different cell subpopulations of the peripheral blood of the donor haplotype as well as in cross-reactivity for different haplotypes. RBC specific cross-reactive antigens attributed to B-G molecules were demonstrated for the B5:B19, B12:B19, and B19:B21 cross-reactions. Cross-reactive antigens detected on RBC and thrombocytes attributable to B-G molecules on both types of cells were demonstrated for the B2:B12, B2:B15, B2:B19, and B2:B21 cross-reactions. In addition, cross-reactive antigens occurring on RBC and WBC were attributed toB-F (or RBC and lymphocyte-expressedB-G loci) and included the B12:B13, B13:B19, and B15:B19 cross-reactions. Several antisera with specificity for B cells purportedly identifying B-L epitopes were found but their numbers were limited and cross-reactivities were not defined. The identities described here may be useful in understandingB haplotype similarities and differences in disease resistance and immune response.

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  1. Agricultural Research Service, Avian Disease and Oncology Laboratory, USDA, 3606 East Mount Hope Road, 48823, East Lansing, Michigan, USA

    Janet E. Fulton, Evelyn E. Young & Larry D. Bacon

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  1. Janet E. Fulton
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  2. Evelyn E. Young
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  3. Larry D. Bacon
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Fulton, J.E., Young, E.E. & Bacon, L.D. ChickenMhc alloantiserum cross-reactivity analysis by hemagglutination and flow cytometry. Immunogenetics 43, 277–288 (1996). https://doi.org/10.1007/BF02440995

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