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The use of immobilized concanavalin a for the separation of alternansucrase from dextransucrase in culture broth ofLeuconostoc mesenteroides NRRL B-1355

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Biotechnology Techniques

Summary

Immobilized concanavalin A has been used to bind a polysaccharide-glucosyltransferase complex fromLeuconostoc mesenteroides NRRL B-1355, which is capable of synthesizing the unusual D-glucan alternan from sucrose. The dextransucrase present in culture fluid passes through a column of immobilized concanavalin A without binding, while the portion of alternansucrase that does bind is eluted using 1-O-methyl α-D-mannopyranoside.

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Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 North University Street, 61604, Peoria, Illinois, U.S.A.

    Gregory L. Cote

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  1. Gregory L. Cote
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The mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned.

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Cote, G.L. The use of immobilized concanavalin a for the separation of alternansucrase from dextransucrase in culture broth ofLeuconostoc mesenteroides NRRL B-1355. Biotechnol Tech 6, 45–48 (1992). https://doi.org/10.1007/BF02438686

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  • DOI: https://doi.org/10.1007/BF02438686

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