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Effect of heat and detergent on glucose-6-phosphatase activity in microsomes isolated from rats of different ages

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Abstract

Hepatic microsomal glucose-6-phosphatase activity in male Fischer rats declines with age. It is unknown whether the phospholipid composition of the microsomal membrane contributes to this age-related decline in enzyme activity. This report compares the effect of two lipid modifiers, heat and detergent, on the activity of glucose-6-phosphatase in microsomes isolated from male Fischer 344 rats of different ages. The heat lability of this enzyme in intact and detergent-disrupted microsomes was not age dependent. These results suggest that the decline in glucose-6-phosphatase activity during liver senescence is not due to lipid changes in the microenvironment of this enzyme that are detectable by thermolability and detergent sensitivity.

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Authors and Affiliations

  1. Department of Anatomy and Histology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, 15261

    Kathleen Dobrosielski-Vergona

  2. Department of Anatomy and Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, 15261

    Kathleen Dobrosielski-Vergona

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  1. Kathleen Dobrosielski-Vergona
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Dobrosielski-Vergona, K. Effect of heat and detergent on glucose-6-phosphatase activity in microsomes isolated from rats of different ages. AGE 7, 67–70 (1984). https://doi.org/10.1007/BF02432203

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  • DOI: https://doi.org/10.1007/BF02432203

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