Summary
A revised and enlarged genetic map of theAgrobacterium tumefaciens C58 chromosome has been produced with the help of plasmid R68.45. Apart from the location of several auxotrophic markers, the map also shows the position of two independent genes,ctu1 andctu2, which, when mutated, block the tumorigenesis of the bacterium. Of these two, onlyctu1 is complemented by the C58 chromosomalvir region cloned by Douglas et al. (1985). The same mutant was complemented by a chromosomal gene or genes located nearleu ofRhizobium meliloti and known to affect the nodulation properties of that bacterium. It has also been observed that C58 tryptophan auxotrophs invariably lose tumorigenicity. Prototrophic revertants and mutants supplied with extra tryptophan for about two weeks after infection produce normal tumours. These investigations suggest that for successful tumorigenesis a continuous supply of tryptophan is needed (to be converted into auxin IAA?) at least during the early stages.
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Miller, I.S., Fox, D., Saeed, N. et al. Enlarged map ofAgrobacterium tumefaciens C58 and the location of chromosomal regions which affect tumorigenicity. Molec. Gen. Genet. 205, 153–159 (1986). https://doi.org/10.1007/BF02428045
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DOI: https://doi.org/10.1007/BF02428045