Abstract
A large plasmid enables its host Agrobacterium tumefaciens to cause tumorous condition in a wide variety of dicotyledonous plants [see Ooms et al. Gene 14:33–50 (1981)]. The location and role of chromosomal genes in this phenomenon are not known. As the first stage in studying this aspect, a project was initiated to investigate the chromosomal genetics of the bacterium. R68.45, a P group plasmid, was chosen as a transmission agent. After a preliminary assessment it was decided to use C58 as a standard strain to carry out the mapping. The plasmid itself, as judged by the presence of antibiotic markers, appears to be stable in a. tumefaciens; its ability to promote chromosomal mobilisation, however, remains only in 60–80% transconjugants. Good Agrobacterium donors are capable of transferring chromosomal genes at a frequency varying between 10-5 to 10-6 per recipient. The recombinants are stable even under non-selective conditions. A linear linkage map consisting of 16 markers was built using coinheritance frequencies obtained from 21 four-point crosses.
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References
Beringer JE, Hoggan SA, Johnston AWB (1978) Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination. J Gen Microbiol 104:201–207
Bernaerts MJ, De Ley J (1963) A biochemical test for crown gall bacteria. Nature 197:406–407
Chilton MD, Currier TC, Farrand SK, Bendich AJ, Gordon MP, Nester EW (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA detected in Crown-gall tumors. Proc Natl Acad Sci USA 71:3672–3676
Chilton MD, Drummond MH, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of Crown-gall tumorigenesis. Cell 11:263–271
Clowes RC, Hayes W (1968) Experiments in microbial genetics. Balck-well Scientific Publications, Oxford
Drummond M (1979) Crow gall disease. Nature 281:343–347
Garfinkel DJ, Nester EW (1980) Agrobacterium tumefaciens mutants affected in Crown gall tumorigenesis and Octopine Catabolism. J Bacterial 144:732–743
Grinter NJ (1981) Analysis of chromosome mobilization using hybrids between plasmids RP4 and a fragment of bacteriophage carrying Is-1. Plasmid 5:267–276
Hass D, Holloway BW (1976) R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Molec Gen Genet 144:243–251
Haas D, Holloway BW (1978) Chromosome mobilization by the R plasmid R68.45: a tool in Pseudomonas genetics. Molec Gen Genet 158:229–237
Hamada SE, Luckey JP, Farrand SK (1979) R-plasmid mediated chromosomal gene transfer in Agrobacterium tumefaciens. J Bacteriol 139:280–286
Hamilton HR, Fall MZ (1971) The loss of tumor-initiating ability in Acrobacterium tumefaciens by induction at high temperature. Experimentia 27:229–230
Holloway BW (1979) Plasmids that mobilize the bacterial chromosome. Plasmid 2:1–19
Hopwood DA (1972) Genetic analysis in micro-organisms. In: JR Norris, DW Ribbons (eds) Methods in microbiology, vol 7B, pp 30–158
Langley RA, Kado CI (1972) Studies on Agrobacterium tumefaciens. Conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine and relationship of Agrobacterium tumefaciens mutants to crown-gall inducation. Mut Res 14:277–286
Ooms G, Hooykaas PJJ, Moolenaar G, Schiperoot RA (1981) Crown gall plant tumours of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions. Gene 14:33–50
Stanisich VA, Holloway BW (1972) a mutant sex factor of Pseudomonas aeruginosa. Genet Res 19:91–108
Thomashow MF, Nutter R, Montoya AL, Gordon MP, Nester EW (1980) Integration and organization of Ti plasmid sequences in Crown gall tumors. Cell 19:729–739
Vogel HL, Bonner DM (1956) Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem 218:97–106
Zambryski P, Holsters M, Kruger K, Depicker A, Schell J, Van Montagu M, Goodman HM (1980) Tumor DNA structure in plant cells transformed by A. tumefaciens. Science 209:1385–1391
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Bryan, J., Saeed, N., Fox, D. et al. R68.45 mediated chromosomal gene transfer in Agrobacterium tumefaciens . Arch. Microbiol. 131, 271–277 (1982). https://doi.org/10.1007/BF00405892
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DOI: https://doi.org/10.1007/BF00405892