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Hexokinase microheterogeneity in rabbit red blood cells and its behaviour during reticulocytes maturation

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Abstract

Hexokinase in rabbit reticulocytes is present in two molecular forms (hexokinase Ia and Ib) separable by ion-exchange chromatography on DE-52 columns. By the use of ion-exchange HPLC we have been able to show that the isozymic form we previously called hexokinase la can be resolved into two peaks of activity one of which is (Ia) soluble, the other (Ia*) particulate. Hexokinase Ia* can be solubilized by detergents like saponine and Triton X-100 and disappears during ‘in vivo’ reticulocytes maturation. This new hexokinase micro-heterogeneity is not caused by different oxidized forms of the enzyme nor influenced by the presence of proteolytic inhibitors during lysate preparation.

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Authors and Affiliations

  1. Istituto di Chimica Biologica, Universitá degli Studi, Via Saffi, 2, 61029, Urbino, Italy

    Vilberto Stocchi, Mauro Magnani, Giovanni Piccoli & Giorgio Fornaini

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  1. Vilberto Stocchi
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  2. Mauro Magnani
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  3. Giovanni Piccoli
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  4. Giorgio Fornaini
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Stocchi, V., Magnani, M., Piccoli, G. et al. Hexokinase microheterogeneity in rabbit red blood cells and its behaviour during reticulocytes maturation. Mol Cell Biochem 79, 133–136 (1988). https://doi.org/10.1007/BF02424555

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  • DOI: https://doi.org/10.1007/BF02424555

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