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Changes in Hemoglobin Isoforms in the Peripheral Blood of Rats with Experimental Posthemorrhagic Anemia

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Bulletin of Experimental Biology and Medicine Aims and scope

Six hemoglobin isoforms were identified in the peripheral blood of male Wistar rats by PAAG electrophoresis. Erythrocytes can be separated into 6 fractions by fractional centrifugation; the fractions differ by the content of reticulocytes and cells with fetal hemoglobin. Cells in each fraction contain one light and one heavy hemoglobin isoforms, and their combination is specific to each fraction. Changes in the hemoglobin isoforms in the whole blood in case of blood loss can be associated with changes in physical and chemical properties of circulating erythrocytes, as well as with the formation of various cell populations resulting from activated erythropoiesis.

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References

  1. Brilliant SA, Yshkov BG. The study hemoglobine spectrum of rats bone marrow with post-hemorrhagic anemia. Vestn. Ural. Med. Akad. Nauki. 2018;15(4):570-576. https://doi.org/10.22138/2500-0918-2018-15-4-570-576

    Article  Google Scholar 

  2. Kriventsev YuA, Kriventseva LA. Hemoglobins of the human as diagnostic markers. Nauch. Obozrenie. Med. Nauki. 2018;(1):16-20. Russian.

    Google Scholar 

  3. Manchenko E.A., Kozlova E.K., Sergunova V.A., Chernysh A.M. Homogeneous Deformation of Native Erythrocytes During Long-Term Storage. Obshchyaya Reanimatologiya. 2019;15(5):4-10. https://doi.org/10.15360/1813-9779-2019-5-4-10

    Article  Google Scholar 

  4. Ostroushko AA, Danilova IG, Brilliant SA, Gette IF. Application of nanocluster iron–molybdene polyoxometalates for correction of experimental posthemorrhagic anemia. Nanotechnologies in Russia. 2019;14(3-4):159-164.

    Article  CAS  Google Scholar 

  5. Sumin MN, Rezaikin AV, Yushkov BG. Hemoglobin heterogeneity under conditions of abnormal erythropoiesis. Bull. Exp. Biol. Med. 2003;135(6):563-565. https://doi.org/10.1023/a:1025477202093

    Article  CAS  PubMed  Google Scholar 

  6. Topunov AF, Petrova NE. Hemoglobin: evolution, distribution, and heterogeneity. Uspekhi Biol. Khimii. 2001;41:199-228. Russian.

    CAS  Google Scholar 

  7. Shamratova VG, Usmanova SR, Isaeva EE. Role of fetal hemoglobin in adaptation to hypoxia in case of limited physical activity. Biotechnology-from science to practice. Ibragimov RI, ed. Ufa, 2014. P. 81-85. Russian.

  8. Yushkov BG, Brilliant SA. Distribution various isoforms of hemoglobin in rat red blood cells. Ross. Fiziol. Zh. 2020;106(10):1312-1320. https://doi.org/10.31857/S0869813920090095

    Article  Google Scholar 

  9. Datta P, Chakrabarty S, Chakrabarty A, Chakrabarti A. Membrane interactions of hemoglobin variants, HbA, HbE, HbF and globin subunits of HbA: Effects of aminophospholipids and cholest erol. Biochim. Biophys. Acta. 2008;1778(1):1-9. https://doi.org/10.1016/j.bbamem.2007.08.019

    Article  CAS  PubMed  Google Scholar 

  10. Gell DA. Structure and function of haemoglobins. Blood Cells Mol. Dis. 2018;70:13-42. https://doi.org/10.1016/j.bcmd.2017.10.006

    Article  CAS  PubMed  Google Scholar 

  11. Grispo MT, Natarajan C, Projecto-Garcia J, Moriyama H, Weber RE, Storz JF. Gene duplication and the evolution of hemoglobin isoform diff erentiation in birds. J. Biol. Chem. 2012;287(45):37647-37658. https://doi.org/10.1074/jbc.M112.375600

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Opazo JC, Hoffmann FG, Natarajan C, Witt CC, Berenbrink M, Storz JF. Gene turnover in the avian globin gene families and evolutionary changes in hemoglobin isoform expression. Mol. Biol. Evol. 2015;32(4):871-887. https://doi.org/10.1093/molbev/msu341

    Article  CAS  PubMed  Google Scholar 

  13. Smith RD, Li J, Noguchi CT, Schechter AN. Quantitative PCR analysis of HbF inducers in primary human adult erythroid cells. Blood. 2000;95(3):863-869.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Yekaterinburg, Russia

    B. G. Yushkov & S. A. Brilliant

  2. Insitute of Natural Sciences and Mathematics, B. N. Yeltsin Ural Federal University, Yekaterinburg, Russia

    B. G. Yushkov & A. S. Minin

  3. Institute of Cell Technologies in Medicine, Yekaterinburg, Russia

    B. G. Yushkov & S. A. Brilliant

Authors
  1. B. G. Yushkov
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  2. S. A. Brilliant
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  3. A. S. Minin
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Correspondence to S. A. Brilliant.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 4, pp. 424-428, April, 2021

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Yushkov, B.G., Brilliant, S.A. & Minin, A.S. Changes in Hemoglobin Isoforms in the Peripheral Blood of Rats with Experimental Posthemorrhagic Anemia. Bull Exp Biol Med 171, 421–424 (2021). https://doi.org/10.1007/s10517-021-05241-0

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  • DOI: https://doi.org/10.1007/s10517-021-05241-0

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