Skip to main content
Log in

Comparative assessment of toxicity of phenol, formaldehyde, and industrial wastewater to aquatic organisms

  • Published:
Water, Air, and Soil Pollution Aims and scope Submit manuscript

Abstract

The toxicity of pure phenol, formaldehyde, and industrial wastewater, containing phenol and formaldehyde, from a resin production plant was evaluated using aquatic organisms from different taxonomic groups. Test organisms included mixed bacterial culture, unicellular green algaeScenedesmus quadricauda (Turp.) Breb., crustaceaDaphnia pulex de Geer (daphnids), and fishOncorhynchus mykiss Call, 1990 (rainbow trout).

Formaldehyde was found to be more toxic to the mixed bacterial culture (120h EC50 = 34.1 mg L−1), algae (24h EC50 = 14.7 mg L−1), and crustacea (48h EC50 = 5.8 mg L−1) than phenol. Phenol proved to be more toxic to fish (48h LC50 = 13.1 mg L−1) than to the mixed bacterial culture (120h EC50 = 510 mg L−1), algae (24h EC50 = 403 mg L−1), and crustacea (48h EC50 = 25 mg L−1). The toxicity of the industrial wastewater to the mixed bacterial culture, algae, and crustacea was caused mainly by formaldehyde, but for fish the presence of phenol in the wastewater proved to be the significant reason for toxicity. Differences in sensitivity of the selected test organisms were also observed, with fish and crustacea being the most sensitive species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Alsop, G., Waggy, G. T. and Conway, R. A.: 1980,J. Water Pollut. Control Fed. 52, 2452.

    CAS  Google Scholar 

  • APHA-AWWA-WEF: 1992,Standard Methods for the Examination of Water and Wastewater, 18 Edition, Washington, D.C.

  • Baudo, R.: 1987,Mem. Ist. Ital. Idrobiol. 45, 461.

    Google Scholar 

  • Bitton, G. and Dutka, J. B.: 1986,Toxicity Testing using Microorganisms, vol. 1, CRC Press, Boca Raton, Florida.

    Google Scholar 

  • Block, J. C., Bauda, P. and Reteuna, C.: 1989, ‘Ecotoxicity Testing Using Aquatic Bacteria’, in A. Boudou and F. Ribeyre (eds.),Aquatic Ecotoxicology: Fundamental Concepts and Methodologies, vol. II, CRC Press, Boca Raton, Florida.

    Google Scholar 

  • Bringmann, G. and Kühn, R.: 1960,Gesundheits-Ingenieur 11, 337.

    Google Scholar 

  • Bringmann, G. and Kuhn, R.: 1980,Water Res. 14, 231.

    Article  CAS  Google Scholar 

  • Bringmann, G. and Kuhn, R.: 1982,Z. Wasser Absvasser Forsch. 15, 1.

    CAS  Google Scholar 

  • Curtis, C., Lima, A., Lozano, S. J. and Veith, G. D.: 1982, inAquatic Toxicology and Hazard Assessment, Proceedings of the fifth symposium on Aquatic Toxicology, ASTM STP 766, Philadelphia, 170.

  • Dorward, J. E. and Barisas, B. G.: 1984,Environ. Sci. Technol. 18, 967.

    Article  CAS  Google Scholar 

  • Grahl, K.: 1983,Acta Hydrochimica et Hydrobiologia 11, 137.

    CAS  Google Scholar 

  • Hunter, J. B.: 1978,Wat. Pollut. Control 77, 384.

    CAS  Google Scholar 

  • International Organization of Standardisation: 1989, ISO Water Quality - Determination of Biochemical Oxygen Demand after 5 days (BOD5) — Dilution and Seeding Method, ISO 5815:1989(E).

  • International Organization of Standardisation: 1989, ISO Water Quality — Determination of Chemical Oxygen Demand, ISO 6060:1989(E).

  • International Organization of Standardisation: 1991, ISO Water Quality - Evaluation in an Aqueous Medium of the “Ultimate” Aerobic Biodegradability of Organic Compounds — Method by Determining the Oxygen Demand in a Closed Respirometer, ISO 9408:1991(E).

  • Leithe, W.: 1974,Die Analyse der Luft and ihrer Verunreinigungen in der freien Atmosphäre und am Arbeitsplatz, Wissenschaftliche Verlagsgesellschaft MBH, Stuttgart.

    Google Scholar 

  • Liebmann, H.: 1960,Handbuch der Frischwasser und Abwasserbiologie,II, R. Oldenbourg, München.

    Google Scholar 

  • Mc Feters, G. A., Bond, P. J., Olson, S. B. and Tchan, Y. T.: 1983,Water Res. 17, 1757.

    Article  Google Scholar 

  • Offhaus, K.: 1973,Münchner Beitr Abwasser-, Fisch.- Flussbiol. 24, 169.

    CAS  Google Scholar 

  • Organisation for Economic Cooperation and Development: 1982,Daphnia sp., Acute Immobilisation Test and Reproduction Test, 202, Paris.

  • Peukert, V.: 1967,Fortschr. Wasserchem. ihrer Grenzgeb. 5, 323.

    Google Scholar 

  • Phipps, G. L., Harden, M. J., Leonard, E. N., Roush, T. H., Spehar, D. L., Stephan, C. E., Pickering, Q. H. and Buikema, A. L.: 1984,J. Water Pollut. Control Fed. 56, 725.

    CAS  Google Scholar 

  • Qureshi, A. A., Flood, K. W., Thompson, S. R., Janhurst, S. M., Inniss, C. S. and Rokosh, D. A: 1982,Aquatic Toxicology and Hazard Assessment, Proceeding of the fifth symposium on Aquatic Toxicology, ASTM STP 766, Philadelphia, 179.

  • Rippen, G.: 1984,Handbuch der- Umweltchemikalien, Physikalisch-chemische und ökotoxikologische Daten ausgewälhlter cheinischer Stofje, Ecomed, Landsberg/Lech.

    Google Scholar 

  • Shigeoka, T., Sato, Y., Takeda, Y., Yoshida, K. and Yamauchi, F.: 1988,Environ. Toxicol. Chem. 7, 847.

    CAS  Google Scholar 

  • Skibba, W. D.: 1982,Ausgewählte Methoden der Wasseruntersuchung, Biologische, mikrobiologische und toxikologische Methoden: Band II., VEB Gustav Fischer Verlag Jena.

  • Sprague, J. B.: 1969,Water Res. 3, 793.

    Article  CAS  Google Scholar 

  • Statistical Support Staff Computer Sciences Corporation: 1988, Users Guide for a Computer Program for Probitts Analysis of Data from Acute and Short-term Chronic Toxicity Tests with Aquatic Organisms. The Biological Methods Branch, Environmental Monitoring and Support Laboratory, United States Environmental Protection Agency, Cincinnati.

  • Tümpling von, W.: 1972,Fortschr. Wasserchem. ihrer Grengeb. 14, 205.

    Google Scholar 

  • Verschueren, K.: 1977,Handbook of Environmental Data on Organic Chemicals, Van Nostrand Reinhold Company, New York.

    Google Scholar 

  • Vittozzi, L. and De Angelis, G.: 1991,Aquat. Toxicol. 19, 167.

    Article  CAS  Google Scholar 

  • Wall, T. M. and Hanmer, R. W.: 1987,J. Water Pollut. Control Fed. 59, 7.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tišler, T., Zagorc-Končan, J. Comparative assessment of toxicity of phenol, formaldehyde, and industrial wastewater to aquatic organisms. Water Air Soil Pollut 97, 315–322 (1997). https://doi.org/10.1007/BF02407469

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02407469

Key words

Navigation