Abstract
Stylar proteins involved in the self-incompatible (SI) response ofLycopersicon hirsutum have been identified and mapped to the locus that controls SI (S locus).L. esculentum, a self-compatible (SC) species of cultivated tomato, does not display these proteins. Hybrids between SCL. esculentum and SIL. hirsutum are self-sterile despite these individuals bearing pollen containing theS allele ofL. esculentum. In progeny derived from backcrossing the hybrids toL. esculentum, there was a strong correlation between the presence of theS allele fromL. hirsutum and self-infertility. However, this relationship was uncoupled in a number of backcross (BC) progeny. The SI response appeared to be nonexistent in two self-fertile BC individuals that were heterozygous for theS allele ofL. hirsutum, based on Mendelian segregation of a tightly linked DNA marker,CD15, in selfed progeny. Among these progeny self-fertile individuals that were homozygous for theL. hirsutum allele of the linked marker were also determined to be homozygous for anS-related protein ofL. hirsutum through test crosses withL. esculentum. Therefore, plants were produced that were homozygous for a functionalS allele but were self-fertile. This result and other evidence suggest that theS-related proteins are not sufficient to elicit a self-incompatible response inL. esculentum and that there is a mutation(s) inL. esculentum somewhere other than theS locus that leads to self-compatibility.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ai, Y., Kron, E., and Kao, T-h. (1991).S-alleles are retained and expressed in a self-compatible cultivar ofPetunia hybrida.Mol. Gen. Genet. 230353.
Bernatzky, R. (1993). Genetic mapping and protein product diversity of the self-incompatibility locus in wild tomato (Lycopersicon peruvianum).Biochem. Genet. 31173.
Bernatzky, R., and Miller, D. D. (1994). Self incompatibility is codominant in intraspecific hybrids of self-compatible and self-incompatibleLycopersicon peruvianum andL. hirsutum based on protein and DNA marker analysis.Sex. Plant Reprod. 7297.
Bernatzky, R., and Schilling, A. (1992). Methods for Southern blotting and hybridization. In Osborn, T. C., and Beckmann, J. S. (eds.),Plant Genomes: Methods for Genetic and Physical Mapping Kluwer Academic, Dordrecht, The Netherlands.
Bernatzky, R., and Tanksley, S. D. (1986). Genetics of actin-related sequences in tomato.Theor. Appl. Genet. 72314.
Chetelat, R. T., and DeVerna, J. W. (1991). Expression of unilateral incompatibility in pollen ofLycopersicon pennellii is determined by major loci on chromosomes 1, 6 and 10.Theor. Appl. Genet. 82704.
Dana, M. N., and Ascher, P. D. (1986a). Sexually localized expression of pseudo-self compatibility (PSC) inPetunia × hybrida hort. 1. Pollen inactivation.Theor. Appl. Genet. 71573.
Dana, M. N., and Ascher, P. D. (1986b). Sexually localized expression of pseudo-self compatibility (PSC) inPetunia × hybrida hort. 2. Stylar inactivation.Theor. Appl. Genet. 71578.
de Nettancourt, D. (1977).Incompatibility in Angiosperms Springer-Verlag, New York.
Flaschenriem, D. R., and Ascher, P. D. (1979).S-allele discrimination in styles ofPetunia hybrida bearing stylar conditioned pseudo-selfcompatibility.Theor. Appl. Genet. 5523.
Jahnen, W., Lush, W. M., and Clarke, A. E. (1989a). Inhibition of pollen tube growth by isolated S-glycoproteins ofNicotiana alata.Plant Cell 1501.
Jahnen, W., Batterham, M. P., Clarke, A. E., Moritz, R. L., and Simpson, R. J. (1989b). Identification, isolation, and N-terminal sequencing of style glycoproteins associated with self-incompatibility inNicotiana alata.Plant Cell 1493.
Kao, T-h., and Huang, H. (1994). Gametophytic self-incompatibility: A mechanism for self/nonself discrimination during sexual reproduction.Plant Physiol. 105461.
Lee, H.-S., Huang, S., and Kao, T-h. (1994).S-proteins control rejection of incompatible pollen inPetunia inflata.Nature 367560.
Liedl, B. E., Liu, S.-C., Esposito, D., and Mutschler, M. A. (1993). Identification and mapping of S in a self-compatible F2 population ofL. esculentum × L. pennellii.Rep. Tomato Genet. Coop. 4333.
Martin, F. W. (1961). The inheritance of self-incompatibility in hybrids ofLycopersicon esculentum Mill. ×L. chilense Dun.Genetics 461443.
Martin, F. W. (1968). The behavior ofLycopersicon incompatibility alleles in an alien genetic millieu.Genetics 60101.
Martin, G. B., Brommonschenkel, S. H., Chungwongse, J., Frary, A., Ganal, M. W., Spivey, R., Wu, T., Earle, E. D., and Tanksley, S. D. (1993). Map-based cloning of a protein kinase gene conferring disease resistance in tomato.Science 2621432.
Matton, D. P., Nass, N., Clarke, A. E., and Newbigin, E. (1994). Self-incompatibility: How plants avoid illegitimate offspring.Proc. Natl. Acad. Sci. USA 911992.
McClure, B. A., Haring, V., Ebert, P. R., Anderson, M. A., Simpson, R. J., Sakiyama, F., and Clarke, A. E. (1989). Style self-incompatibility gene products ofNicotiana alata are ribonucleases.Nature 342955.
McClure, B. A., Gray, J. E., Anderson, M. A., and Clarke, A. E. (1990). Self-incompatibility inNicotiana alata involves degredation of pollen rRNA.Nature 347757.
Murfett, J., Atherton, T. L., Mou, B., Gasser, C. S., and McClure, B. A. (1994). S-RNase expression in transgenicNicotiana causes S-allele-specific pollen rejection.Nature 367563.
Rick, C. M. (1988). Molecular markers as aids for germplasm management and use inLycopersicon.HortSci. 2355.
Rick, C. M., and Chetelat, R. T. (1991). The breakdown of self-incompatibility inLycopersicon hirsutum. In Hawkes, Lester, Nee, and Estrada (eds.),Solanaceae III: Taxonomy, Chemistry, Evolution, Royal Botanic Gardens Kew and Linnean Society of London, pp. 253–256.
Rivers, B. A., and Bernatzky, R. (1994). Protein expression of a self-incompatible allele fromLycopersicon peruvianum: Introgression and behavior in a self-incompatible background.Sex. Plant Reprod. 7357.
Rivers, B. A., Bernatzky, R., Robinson, S. J., and Jahnen-Dechent, W. (1993). Molecular diversity at the self-incompatibility locus is a salient feature in natural populations of wild tomato (Lycopersicon peruvianum).Mol. Gen. Genet. 238419.
Royo, J., Kunz, C., Kowyama, Y., Anderson, M. A., Clarke, A. E., and Newbigin, E. (1994). Loss of a histidine residue at the active site of S-ribonuclease leads to self compatibility inLycopersicon peruvianum.Proc. Natl. Acad. Sci. USA 916511.
Sims, T. L. (1993). Genetic regulation of self-incompatibility.Crit. Rev. Plant Sci. 12129.
Tanksley, S. D., and Loaiza-Figueroa, F. (1985). Gametophytic self-incompatibility is controlled by a single major locus on chromosome 1 inLycopersicon peruvianum.Proc. Natl. Acad. Sci. USA 825093.
Thompson, R. D., and Kirsch, H.-H. (1992). TheS-locus of flowering plants: When self-rejection is self interest.Trends Genet. 8383.
Thompson, R. D., Uhrig, H., Hermsen, J. G. T., Salamini, F., and Kaufmann, H. (1991). Investigation of a self-compatible mutation inSolanum tuberosum clones inhibiting S-allele activity in pollen differentially.Mol. Gen. Genet. 226283.
Woodward, J. R., Bacic, A., Jahnen, W., and Clarke, A. E. (1989). N-linked glycan chains onS-allele-associated glycoproteins fromNicotian alata Plant Cell 1511.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bernatzky, R., Glaven, R.H. & Rivers, B.A. S-related protein can be recombined with self-compatibility in interspecific derivatives ofLycopersicon . Biochem Genet 33, 215–225 (1995). https://doi.org/10.1007/BF02401852
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02401852