Abstract
The57Fe Mössbauer spectra of concentrated hemoglobin (Hb) of normal subjects and six patients with Paraxysmal Nocturnal Hemoglobinuria (PNH) were studied at 300°K and 77 K. PNH is a very rare autoimmune hematological disease. The possibility of structural alterations of Hb induced by, or as part of the altered PNH-red cell membrane was the objective of this study. The Mössbauer parameters of the Hb of the normal subjects, both at 300 K and at 77 K, are identical to values previously reported. The PNH-Hb spectra show clear differences. They are wider and more asymmetric. At 77 K an extra doublet grows in with an isomer shift of 0.425 mm/sec. and a quadrupolar splitting, of 1.951 mm/sec. The other two doublets have δ′s and ΔQ's slightly, but significantly, different from the corresponding values for normal Hb. These results are rationalized in terms of a population of Hb molecules with structures varying very slightly in a narrow range. The spread in structures manifests itself in a wider and more asymmetric Mössbauer spectrum.
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R. Zamorano-Ulloa and H. Yee-Madeira, to be published.
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Zamorano-Ulloa, R., Yee-Madeira, H., Flores-Llamas, H. et al. A Mössbauer study of hemoglobin in paroxysmal nocturnal hemoglobinuria. Hyperfine Interact 67, 611–617 (1991). https://doi.org/10.1007/BF02398209
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DOI: https://doi.org/10.1007/BF02398209