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Poly(methylglutamate)-coated surfaces in HPLC and CE

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Thermal polymerization of the N-carboxyanhydride of glutamic acid-5-methylester was used to coat microparticulate silica and the surface of capillaries with poly(methylglutamate) (PMG). By increasing the thickness of the PMG layer the hydrophobicity of the stationary phases could be increased. However, total shielding of surface silanols was not achieved. The PMG-coated silicas for HPLC showed unexpected and strange behavior in the chromatography of proteins. Nevertheless, capillaries coated with PMG proved to be excellent for highly efficient CE separations of proteins at medium pH values.

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  1. Angewandte Physikalische Chemie, Universität des Saarlandes, 6600, Saarbrücken, FRG

    D. Bentrop, J. Kohr & H. Engelhardt

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  1. D. Bentrop
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  2. J. Kohr
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  3. H. Engelhardt
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Bentrop, D., Kohr, J. & Engelhardt, H. Poly(methylglutamate)-coated surfaces in HPLC and CE. Chromatographia 32, 171–178 (1991). https://doi.org/10.1007/BF02325022

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