Summary
A pragmatic test procedure for comparison and evaluation of reversed phases is described. The differences in retention between RP8 and RP18 can be compensated for by adjustment of the eluent composition. A 10% increase in the water content doubles the k′ values, as does the exchange of an RP8 with an RP18 column. It is possible to differentiate between the two columns with the pair ethylbenzoate and toluene. Under stand-ard conditions (methanol-water, 55–45, v/v; 49–51, w/w) with RP8 ethylbenzoate is always eluted together or after toluene, whereas with RP18 it is always eluted in front of toluene.
With the same eluent composition the suitability of stationary phases for the separation of basic solutes can be evaluated. With “good” phases—symmetrical peaks for basic solutes—aniline is always eluted before phenol and the peak asymmetry relationship of the aniline and phenol peak is less than 1.3. With such “good” stationary phases the retention of bases is independent of sample size if the linear capacity of 0.1 mg sample/g stationary phase is not exceeded. The test can also be used to study column stability towards hydrolysis.
Similar content being viewed by others
References
J. J. Kirkland, J. Chromatogra. Sci.9, 206 (1971).
G. A. Horvard, A. J. P. Martin, Biochem. J.46, 215 (1951).
R. E. Majors, LC&GC Magazine6, 298 (1988).
Cs. Horvath, W. Melander, in:Cs. Hovarth, ed., High Performance Liquid Chromatography, Vol. 2, Academic Press, New York 1980.
H. Engelhardt, B. Dreyer, H. Schmidt, Chromatographia16, 11 (1982).
K. K. Unger, Porous Silica, Elsevier, Amsterdam 1979.
B. L. Karger, J. N. Le Page, N. Tanaki, in:Cs. Horvath, ed., High Performance Liquid Chromatography, Vol. 1, Academic Press, New York 1980.
T. Daldrup, B. Kardel, Chromatographia18, 81 (1984).
I. Halasz, G. Görlitz, Angew. Chem.94, 50 (1982).
B. A. Bristow, J. H. Knox, Chromatographia10, 279 (1979).
J. C. Giddings, Dynamics of Chromatography, Dekker, New York 1965.
L. R. Snyder, Anal. Chem.39, 698 (1967).
L. R. Snyder, Principles of Adsorption Chromatography, Dekker, New York 1968.
K. Karch, I. Sebastian, I. Halasz, H. Engelhardt, J. Chromatogr.122, 171 (1976).
H. Engelhardt, G. Ahr, Chromatographia14, 227 (1981).
P. Jandera, J. Chromatogr.314, 14 (1984).
D. Morel, J. Serpinet, J. M. Letoffe, P. Claudy, Chromatographia22, 103 (1986).
A. Groß, Master Thesis, Saarbrücken 1985.
A. Wilhelm, Master Thesis, Saarbrücken 1985.
M. L. Miller, R. W. Linton, S. G. Bush, J. W. Jorgenson, Anal. Chem.56, 2205 (1984).
B. A. Bidlingmeyer, J. K. Del Rios, J. Korpl, Anal. Chem.54, 442 (1982).
J. Köhler, J. J. Kirkland, J. Chromatogr.352, 1, 275 (1986).
E. Meyer, H. Engelhardt, Z. anal. Chem.333, 734 (1989).
H. Engelhardt, H. Löw, W. Eberhardt, M. Mauß, Chromatographia27, 335 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Engelhardt, H., Jungheim, M. Comparison and characterization of reversed phases. Chromatographia 29, 59–68 (1990). https://doi.org/10.1007/BF02261141
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02261141