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Correlation between retention and the C18 silica surface coverage in reversed-phase liquid chromatography

  • Late Papers from the 17th International Symposium on Chromatography, Vienna, Austria, September 25–30, 1988
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Summary

Members of three homologous series and five non-homologous solutes with various functional groups were chromatographed on a series of well-characterized C18 reversed stationary phases with a range of methanol-water mobile phases. Measured capacity factors of solutes were correlated with the concentration of C18 ligands on the stationary phase. A linear relationship with the slope depending on a solute molecular structure and the volume fraction of methanol in the mobile phase was obtained. A method for the evaluation of phase ratio is also proposed.

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Authors and Affiliations

  1. Institute of Microbiological Processes and Applied Chemistry, Faculty of Technology, University of Novi Sad, Bul. AVNOJa 1, 21000, Novi Sad, Yugoslavia

    S. M. Petrović & S. M. Lomić

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  1. S. M. Petrović
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  2. S. M. Lomić
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Petrović, S.M., Lomić, S.M. Correlation between retention and the C18 silica surface coverage in reversed-phase liquid chromatography. Chromatographia 27, 378–384 (1989). https://doi.org/10.1007/BF02321287

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  • DOI: https://doi.org/10.1007/BF02321287

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