Skip to main content
SpringerLink
Log in

The Biolistic® PDS-1000/He device

  • Special Section On Particle Bombardment
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

This paper describes the design, operation, and performance of the Biolistic® PDS-1000/He device, which is used to transform living organisms with foreign DNA. DNA is delivered to cells in association with microscopic metal particles, called microcarriers, that are propelled at high velocity towards target tissues. The microcarriers are accelerated on a plastic cylinder, called a macrocarrier, which is driven by a shock wave of helium gas. The effectiveness of the PDS-1000/He device was tested by bombarding tobacco cell suspension cultures with microcarriers that were coated with plasmid DNA containing the B-glucuronidase (GUS) and neomycin phosphotransferase II (NPTII) genes. Two days after bombardment, there were 6835 ± 594 cell clusters per petri plate that expressed the GUS gene. Kanamycin resistant colonies were observed 6 to 8 weeks after bombardment, at a rate of 838 ± 134 colonies per bombarded plate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Russell JA, Roy MK & Sanford JC (1992a) Physical trauma and tungsten toxicity reduce the efficiency of biolistic transformation. Plant Physiol. 98: 1050–1056

    CAS  Google Scholar 

  • Russell JA, Roy MK & Sanford JC (1992b) Major improvements in biolistic transformation of suspension-cultured tobacco cells. In Vitro Cell. Dev. Biol. 28P: 97–105

    CAS  Google Scholar 

  • Sanford JC, Klein TM, Wolf ED & Allen N (1987) Delivery of substances into cells and tissues using a particle bombardment process. Particulate Science and Technology 5: 27–37

    CAS  Google Scholar 

  • Sanford JC (1990) Biolistic plant transformation. Physiol. Plant. 79: 206–209

    Article  CAS  Google Scholar 

  • Sanford JC, DeVit MJ, Russell JA, Smith FD, Harpending PR, Roy MK & Johnston SA (1991) An improved, helium-driven biolistic device. Technique 3: 3–16

    CAS  Google Scholar 

  • Sanford JC, Smith FD & JA Russell (1993) Optimizing the biolistic process for different biological applications. Methods in Enzymology 217: 483–509

    CAS  PubMed  Google Scholar 

  • Smith FD, Harpending PR & Sanford JC (1992) Biolistic transformation of prokaryotes — factors that affect transformation of very small cells. J. Gen. Microbiology 138: 239–248

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. New York State Agricultural Experiment Station, Department of Horticultural Sciences, Cornell University, 14456, Geneva, NY, USA

    Julie Russell Kikkert

Authors
  1. Julie Russell Kikkert
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kikkert, J.R. The Biolistic® PDS-1000/He device. Plant Cell Tiss Organ Cult 33, 221–226 (1993). https://doi.org/10.1007/BF02319005

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02319005

Key words

Search

Navigation