Abstract
Bones cultured for 5 days in a chemically defined medium showed an increased O2 consumption and decreased lactic acid production when ascorbic acid (50 μg/ml) was present in the culture medium. In this and the following experiments these parameters were measured in short-term incubations following tissue culture.
Adding ascorbic acid for only the final day of culture or for the first 4 days and not the final day gave O2 and lactic acid results similar to those obtained when the vitamin was present for all 5 days. Ascorbic acid analogs (50 μg/ml) added for 5 days also exhibited results similar to those for vitamin C.
A preliminary experiment to evaluate the oxidation rate of ascorbic acid in the culture medium established that the vitamin must be replenished daily when cultures are gassed with 20% O2 and more frequently with higher O2 concentrations.
The ascorbic acid-dependent changes in energy metabolism seen in this study suggest that the vitamin may be an important factor in stimulating skeletal aerobic metabolism.
Résumé
Du tissu osseux, cultivé pendant 5 jours dans un milieu chimiquement défini, présente une augmentation de la consommation en O2 et une diminution de la production d'acide lactique lorsqué de l'acide lactique (50 μg/mI) est présent dans le milieu de culture. Ces paramètres ont été mesurés pendant des incubations de courte durée, en cultures de tissus.
En n'ajoutant de l'acide lactique qu'au dernier jour de culture ou pendant les 4 premiers jours, et non le dernier jour, les valeurs en O2 et en acide lactique sont identiques à celles observées lorsque la vitamine est présente pendant 5 jours. Des produits similaires à l'acide ascorbique (50 μg/ml), administrés pendant 5 jours donnent des résultats identiques à ceux de la vitamine C.
Une expérience préliminaire pour évaleur la vitesse d'oxydation de l'acide ascorbique dans le milieu de culture a permis de montrer que la vitamine doit être remplacée quotidiennement lorsque les cultures sont traitées avec 20% d'O2 et plus fréquemment avec des concentrations d'O2 plus élevées.
Les changements dans le métabolisme énergétique, liés à l'acide ascorbique et observés dans cette étude, indiquent que la vitamine pourraît être un facteur important de stimulation du métabolisme squelettique aérobique.
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References
Baker, L. B.: L'effect de l'acide ascorbique sur la proliferation des monocytes. C. R. Soc. Biol. (Paris)121, 427–429 (1936).
Elliott, C. G., andM. D. Smith: Ascorbic acid metabolism and glycolysis in the polymorphonuclear leucocyte of the guinea pig. J. cell comp. Physiol.67, 169–176 (1966).
Follis, R. H., Jr.: Histochemical studies on cartilage and bone. II. Ascorbic acid deficiency. Bull. Johns Hopk. Hosp.89, 9–20 (1951).
Jeffrey, J. J., andG. R. Martin: The role of ascorbic acid in the biosynthesis of collagen. I. Ascorbic acid requirement by embryonic tibia in tissue culture. Biochim. biophys. Acta (Amst.)121, 269–280 (1966).
Lardy, H. A., andH. Wellman: Oxidative phosphorylations: role of inorganic phosphate and acceptor systems in control of metabolic rates. J. biol. Chem.195, 215–224 (1952).
Lundholm, L., E. Mohme-Lundholm, andN. Vamos: Lactic acid assay with L(+) lactic acid dehydrogenase from rabbit muscle. Acta physiol. scand.58, 243–249 (1963).
Massa, V.: Acide ascorbique et métabolisme respiratoire des leucocytes. Bull. Soc. Chim. biol. (Paris)29, 723–734 (1947).
Peck, W. A., andS. J. Birge, Jr.: Response of scorbutic bone cells to ascorbic acidin vitro. J. clin. Invest.45, 1055–1056 (1966) (Abstract).
Ramp, W. K.: Response of embryonic chick tibias to ascorbic acid in tissue culture. Ph.D. Thesis, University of Kentucky 1967.
Reynolds, J. J.: The effects of ascorbic acid on the growth of chick bone rudiments in chemically defined medium. Exp. Cell Res.42, 178–188 (1966).
Rivers, J. M.: Ascorbic acid in metabolism of connective tissue. N.Y. St. J. Med.65, 1235–1238 (1965).
Roe, J. H., andC. A. Kuether: The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid. J. biol. Chem.147, 399–407 (1943).
Shimizu, Y., D. S. McCann, andM. K. Keech: The effect of ascorbic acid on human dermal fibroblasts in monolayer tissue culture. J. Lab. clin. Med.65, 286–306 (1965a).
———: Human dermal fibroblasts in monolayer tissue culture. Effect of ascorbic acid analogues. J. Lab. clin. Med.66, 659–666 (1965b).
Staudinger, H., K. Krisch, andS. Leonhäuser: Role of ascorbic acid in microsomal electron transport and the possible relationship to hydroxylation reactions. Ann. N. Y. Acad. Sci.92, 195–207 (1961).
Stern, B., M. J. Glimcher, andP. Goldhaber: The effect of various oxygen tensions on the synthesis and degradation of bone collagen in tissue culture. Proc. Soc. exp. Biol. (N.Y.)121, 869–872 (1966).
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This work was supported by the United States Public Health Service predoctoral research fellowship no. 5-Fl-GM-24, 458-03.
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Ramp, W.K., Thornton, P.A. The effect of ascorbic acid on the glycolytic and respiratory metabolism of embryonic chick tibias. Calc. Tis Res. 2, 77–82 (1968). https://doi.org/10.1007/BF02279196
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DOI: https://doi.org/10.1007/BF02279196