Skip to main content
SpringerLink
Log in

Ion-pair LC of carbohydrates at alkaline pH. Separation and retention behaviour of glycoconjugates

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A new technique for the separation of carbohydrates as ion-pairs in strongly alkaline solution is presented. Carbohydrates are weakly acidic and partly present as anions at pH≥12 [1]. They are retained as ion-pairs on polymeric solid phases (PRP-1 and PLRP-S) with a hydrophobic quaternary ammonium counter ion present in the mobile phase. The effects of nature and concentration of mobile phase components on the retention of carbohydrates have been investigated and an ion-pair distribution model is proposed. The influence of temperature indicated no changes in retention mechanism with high counter ion concentration, but the resolution decreased with increasing temperature. Saccharides added to the mobile phase were shown to increase the retention and the selectivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. A. Rendleman, Jr., Adv. Chem. Ser.,1, 17 (1973).

    Google Scholar 

  2. S. C. Churms, J. Chromatogr.,500, 555 (1990).

    Article  Google Scholar 

  3. R. W. Goulding, J. Chromatogr.,103, 229 (1975).

    Article  Google Scholar 

  4. M. Stefansson, D. Westerlund, submitted to Chromatographia (accepted for publication).

  5. R. D. Rocklin, C. A. Pohl, J. Liq. Chromatogr.,6 (9), 1577 (1983).

    Google Scholar 

  6. A. Tilly-Melin, Y. Askemark, K.-G. Wahlund, G. Schill, Anal. Chem.,51, 976 (1979).

    Article  Google Scholar 

  7. C. P. Terweij-Groen, S. Heemstra, J. C. Kraak, J. Chromatogr.161, 69 (1978).

    Article  Google Scholar 

  8. Analytical Applications of Complex Equilibria, J. Inczédy, Ellis Horwood Limited, J. Wiley & Sons Inc. (1967).

  9. H. W. Stuurman, J. Köhler, S.-O. Jansson, A. Litzén, Chromatographia,23, 341 (1987).

    Google Scholar 

  10. P. Mukerjee, K. J. Mysels, NSRDS-NBS,36, (1971).

  11. J. Crommen, J. Chromatogr.,186, 705 (1979).

    Article  Google Scholar 

  12. M. Stefansson, B. Lu, Chromatographia ...

Download references

Author information

Authors and Affiliations

  1. Analytical Pharmaceutical Chemistry, Uppsala University Biomedical Center, P.O. Box 574, 751 23, Uppsala, Sweden

    M. Stefansson & D. Westerlund

Authors
  1. M. Stefansson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Westerlund
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stefansson, M., Westerlund, D. Ion-pair LC of carbohydrates at alkaline pH. Separation and retention behaviour of glycoconjugates. Chromatographia 35, 55–60 (1993). https://doi.org/10.1007/BF02278557

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02278557

Key Words

Search

Navigation