Summary
Evaporative light scattering detectors have, in recent years, gained acceptance in chromatography with dense mobile phases i.e. liquid and supercritical fluid chromatography. In the present work an instrument of this type has been used in packed column supercritical fluid chromatography with carbon dioxide/methanol mixtures. Detector response and signal-to-noise ratios have been determined using squalane as test compound. Nebulizer gas flow, evaporator temperature, photomultiplier sensitivity, and mobile phase composition were found to have an influence on instrument performance. With this type of detector the field of packed column SFC applications can be extended to include non-UV-absorbing substances even when mixed mobile phases or composition gradients are necessary for the separation.
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Upnmoor, D., Brunner, G. Packed column supercritical fluid chromatography with light-scattering detection. I. Optimization of parameters with a carbon dioxide/methanol mobile phase. Chromatographia 33, 255–260 (1992). https://doi.org/10.1007/BF02276191
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DOI: https://doi.org/10.1007/BF02276191