Summary
A method for the calculation of the capacity ratio and concentration-time function, basing on the theory of gradient elution chromatography, is examined by using high-speed liquid chromatography data. It is shown that the average capacity ratio and mobile-phase gradient may be easily calculated with help of experimental data obtained from isocratic elution. Using literature data, the capacity ratios predicted for stepwise elution by means of this method, have been compared with experimental data.
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Jaroniec, M., Borówko, M., Narkiewicz, J. et al. Determination of the capacity ratio and concentration-time function for stepwise elution with binary mobile phase with help of liquid chromatography data obtained from isocratic elution. Chromatographia 12, 29–32 (1979). https://doi.org/10.1007/BF02271596
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DOI: https://doi.org/10.1007/BF02271596