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The use of HPLC for the determination of lipids in biological materials

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Summary

Although high-performance-liquid chromatography has become the standard procedure for many analytical problems, it has been slow to gain ground for lipid analysis. During the last years HPLC has been increasingly applied to the analysis of lipids. In this paper we describe the different methods for the separation and determination of natural neutral and polar lipids.

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References

  1. J. Folch, M. Less, G. H. S. Stanley, J. Biol. Chem.226, 497 (1957).

    PubMed  Google Scholar 

  2. A. Hara, N. S. Radin, Anal. Biochem.90, 420 (1978).

    Article  PubMed  Google Scholar 

  3. A. V. Zhukov, A. G. Vereshchagin, Adv. Lipid Res.18, 247 (1981).

    PubMed  Google Scholar 

  4. M. A. Kalunzy, L. A. Duncan, M.V. Merrit, D. E. Epps, J. Lipid Res.26, 135 (1985).

    PubMed  Google Scholar 

  5. G. Schmitz, G. Assmann, J. Chromatogr.307, 65 (1984).

    PubMed  Google Scholar 

  6. F. M. Helmy, M. H. Hack, J. Chromatogr.374, 61 (1986).

    PubMed  Google Scholar 

  7. B. C. Melnik, J. Hollmann, E. Erler, B. Verhoeven, G. Plewig, J. Invest. Dermatol.92, 231 (1989).

    Article  PubMed  Google Scholar 

  8. K. Aitzetmüller, J. Koch, J. Chromatogr.145, 195 (1978).

    PubMed  Google Scholar 

  9. B. Turner, P. Weinreich, GIT Fachz. Lab.3, 252 (1988).

    Google Scholar 

  10. R. Macrae, L. C. Trugo, J. Dick, Chromatographia15, 476 (1982).

    Article  Google Scholar 

  11. M. S. Manku, J. Chromatogr. Sc.21, 357 (1983).

    Google Scholar 

  12. L. D. Metcalfe, A. A. Schmitz, J. R. Pelka, Anal. Chem.38, 514 (1966).

    Article  Google Scholar 

  13. H. D. Durst, M. Milano, E. J. Kikta, S. A. Connelly, E. Grushka, Anal. Chem.47, 1797 (1975).

    Article  PubMed  Google Scholar 

  14. N. A. Hall, G. W. Lynes, N. M. Hjelm, Clin. Chem.34/6, 1041 (1988).

    Google Scholar 

  15. Y. Antoku, T. Sakai, K. Tsukamota, Clinica Chimica Acta169, 121 (1987).

    Article  Google Scholar 

  16. J. H. Wolf, J. Chromatogr.436, 437 (1989).

    Article  Google Scholar 

  17. B. G. Herzlöf, HRG & CC4, 471 (1981).

    Google Scholar 

  18. L. J. R. Barron, G. Santa Maria, J. Chromatogr.28, 183 (1989).

    Google Scholar 

  19. R. Vercaemst, A. Union, M. Rozzenue, I. De Craene, G. De Backer, M. Komitzer, Artheriosclerosis78, 245 (1989).

    Article  Google Scholar 

  20. S. Shi-Hua Chen, A. Y. Kou, J. Chromatogr.227, 25 (1982).

    PubMed  Google Scholar 

  21. F. B. Jungalwala, J. E. Evans, R. H. Mccluer, Biochem. J.155, 55 (1976).

    PubMed  Google Scholar 

  22. A. Nasner, Lj. Kraus, J. Chromatogr.216, 389 (1981).

    Article  Google Scholar 

  23. K. L. Clay, R. C. Murphy, J. L. Andres, J. Lynch, P. M. Henson, Biochem. Biophys. Res. Commun.121, 815 (1984).

    Article  PubMed  Google Scholar 

  24. G. M. Patton, J. M. Fasulo, S. J. Robins, J. Lipid Res.23, 190 (1982).

    PubMed  Google Scholar 

  25. M. F. Caboni, G. Lercker, A. M. Ghe, J. Chromatogr.315, 223 (1984).

    Article  PubMed  Google Scholar 

  26. F. B. Jungawala, J. Rustom, J. E. Evans, R. H. Mccluer, Biochem. J.145, 517 (1975).

    PubMed  Google Scholar 

  27. H. P. Nissen, H. W. Kreysel J. Chromatogr.278, 29 (1983).

    Google Scholar 

  28. V. L. Hanson, J. Y. Park, T. W. Osborn, R. M. Kiral, J. Chromatogr.205, 393 (1981).

    Article  PubMed  Google Scholar 

  29. M. L. Blank, M. Snyder, J. Chromatogr.273, 415 (1981).

    Google Scholar 

  30. S.S.-H. Chen, A. Y. Kou, H. H. Y. Chen, J. Chromatogr.208, 339 (1981).

    PubMed  Google Scholar 

  31. S. S.-H. Chen, A. Y. Kou, H. H. Y. Chen, J. Chromatogr276, 37 (1983).

    PubMed  Google Scholar 

  32. S. A. Long, P. W. Wertz, J. S. Strauss, D. T. Downing, Arch. Dermatol. Res.277, 284 (1985).

    Article  PubMed  Google Scholar 

  33. M. Sugita, M. Iwamori, J. Evans, R. H. Mccluer, J. T. Dulanev, H. W. Moser, J. Lipid Res.15, 223 (1974).

    PubMed  Google Scholar 

  34. H.-S. Fricke, J. Oehlenschläger, J. Chromatogr.252, 331 (1982).

    Article  Google Scholar 

  35. W. W. Christie, J. Lipid Res.26, 507 (1985)

    PubMed  Google Scholar 

  36. S. H. Y. Wong, J. A. Knight, S. M. Hopfer, O. Zaharia, C. N. Leach, F. W. Sundermann, Clin. Chem.33/2, 214 (1987).

    Google Scholar 

  37. J. A. Knight, S. E. Smith, V. E. Kinder, R. K. Pieper, Clin. Chem.34/6 1107 (1988).

    Google Scholar 

  38. T. Miyazawa, R. Saeki, H. Inala, J. Biolumines. Chemilumines.4 (1), 475 (1989).

    Article  Google Scholar 

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Nissen, H.P., Kreysel, H.W. The use of HPLC for the determination of lipids in biological materials. Chromatographia 30, 686–690 (1990). https://doi.org/10.1007/BF02269744

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