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Comparison of injection-generated gradient and isocratic ion-pair chromatography on short microbore column in HPLC

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Summary

Possibilities of using short microbore columns in trace analysis are presented. Retention of ionizing solutes (catecholamines) is compared using both isocratic ionpair chromatography and injection-generated gradient of a counter ion in the mobile phase. Column efficiency is maintained even with a relatively high sample volume.

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Authors and Affiliations

  1. Institute for Analytical Chemistry, Czechoslovak Academy of Sciences, Leninova 82, 611 42, Brno, Czechoslovakia

    M. Krejči & K. Šlais

  2. Institute of Organic Chemistry, German Academy of Sciences, 1199, Berlin, German Democratic Republic

    A. Kunath

Authors
  1. M. Krejči
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  2. K. Šlais
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  3. A. Kunath
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Krejči, M., Šlais, K. & Kunath, A. Comparison of injection-generated gradient and isocratic ion-pair chromatography on short microbore column in HPLC. Chromatographia 22, 311–315 (1986). https://doi.org/10.1007/BF02268781

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  • DOI: https://doi.org/10.1007/BF02268781

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