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Stepwise elution paper chromatography analysis of fractions of thermically dispersed maize, wheat and sorghum starch

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Summary

By the determination of the number-averaged molecular weights and stepwise elution analysis, ist was found that amylose from maize, wheat and sorghum paste, after precipitation with n-butanol, still contained highmolecular weight fractions of amylopectin, with fragments of the grains. A relatively small amount of lowmolecular-weight pure amylopectin was isolated from the pastes. After extended thermal dispersing of the pastes at 120°C the amount of isolated amylopectin increase in dependence of the type of starch. The molecular weights also increase with the exception of sorghum amylopectin. Extended dispersing of the pastes in the autoclave decreases differently the amounts of the precipitated amylose from the dispersions in dependence of the type of starch, and the molecular weights become smaller.

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References

  1. M. Richter, S. Augustat, F. Schierbaum, “Ausgewählte Methoden der Stärkechemie”, Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, 1968.

    Google Scholar 

  2. L. P. Witnauer, F. R. Senti, M. D. Stern, J. Polymer Sci.16, 1 (1955).

    Article  Google Scholar 

  3. C. J. Stacy, J. F. Foster, S. R. Erlander, Makromolekulare Chem.17, 18 (1956).

    Google Scholar 

  4. R. Geddes, C. T. Greenwood, S. MacKenzie, Carbohydrate Res.1, 71 (1965).

    Article  Google Scholar 

  5. H. Baum, G. A. Gilbert, J. Colloid Sci.11, 428 (1956).

    Article  Google Scholar 

  6. L. M. Gilbert, G. A. Gilbert, S. P. Spragg, “Amylose and Amylopectin from Potato Starch” in “Methods in Carbohydrate Chemistry”, edited byR. L. Whistler, Academic Press, New York and London, 1964; Vol. IV, p. 25.

    Google Scholar 

  7. K. H. Meyer, Angew. Chem.63, 153 (1951).

    Google Scholar 

  8. E. Husemann, H. Bartl, Makromolekulare Chem.18/19, 342 (1956).

    Article  Google Scholar 

  9. J. M. G. Cowie, C. T. Greenwood, J. Chem. Soc. (London), 2862 (1957).

  10. C. T. Greenwood, J. Thomson, J. Chem. Soc. (London), 222 (1962).

  11. J. Muetgeert, Advances Carbohydrate Chem.16, 299 (1961).

    Google Scholar 

  12. P. Hiemstra, W. C. Bus, J. Muetgeer, Starch/Stärke8, 235 (1956).

    Google Scholar 

  13. M. Samec, “Die neuere Entwicklung der Kolloidchemie der Stärke”, Theodor Steikopff, Dresden und Leipzig, 1941; p. 5.

    Google Scholar 

  14. M. Richter, M. Ulmann, Kolloid Z.176, 98 (1961).

    Article  Google Scholar 

  15. M. Čeh, V. Doleček, Č. Stropnik, S. Leskovar, Starch/Stärke32, 345 (1980).

    Google Scholar 

  16. M. Čeh, Č. Stropnik, V. Doleček, S. Leskovar, Starch/Stärke35, 48 (1983).

    Google Scholar 

  17. M. Čeh, Č. Stropnik, S. Leskovar, Starch/Stärke37, 22 (1985).

    Google Scholar 

  18. T. J. Schoch, J. Amer. Chem. Soc.64, 2957 (1942).

    Article  Google Scholar 

  19. T. J. Schoch, “The Starch Fractions” in “Starch and its Derivates”, edited byJ. A. Radley, Chapman and Hall Ltd., London, 1953, Vol. 1, p. 123.

    Google Scholar 

  20. M. Čeh, Č. Stropnik, S. Leskovar, Starch/Stärke37, 415 (1985).

    Google Scholar 

  21. D. French, A. O. Pulley, W. J. Whelan, Starch/Stärke15, 349 (1963).

    Google Scholar 

  22. E. J. Bourne, G. H. Donisson, W. N. Haworth, S. Peat, J. Chem. Soc. (London), 1687 (1948).

  23. M. Čeh, Č. Stropnik, S. Leskovar, Starch/Stärke28, 51 (1976).

    Google Scholar 

  24. M. Čeh, Č. Stropnik, V. Doleček, S. Leskovar, Starch/Stärke34, 85 (1982).

    Google Scholar 

  25. M. Čeh, Č. Stropnik, S. Leskovar, Starch/Stärke30, 151 (1978).

    Google Scholar 

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Čeh, M., Stropnik, Č. & Leskovar, S. Stepwise elution paper chromatography analysis of fractions of thermically dispersed maize, wheat and sorghum starch. Chromatographia 22, 255–260 (1986). https://doi.org/10.1007/BF02268769

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  • DOI: https://doi.org/10.1007/BF02268769

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