Skip to main content
SpringerLink
Log in

Hormone release from cultured luteinized-granulosa cells mimics differences seenin vivo in patients undergoing IVF-ET

  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objectives

Previous research from this laboratory has suggested that a relationship exists between the increase in circulating progesterone concentrations at the time of hCG administration and cycle outcome in patients undergoing IVF. Progesterone (P) increases of threefold or better within the 24-h period surrounding hCG administration appeared to be associated with a higher pregnancy rate. These data suggest a functional difference in the luteinized-granulosa of patients undergoing IVF. To test this hypothesis:

Design

A split-split plot arrangement of treatments with two cell sources under five hormonal stimulations at four time points.

Methods

Luteinized-granulosa cells (LGC) were collected from patients with either a normal increase (⩾threefold = NC) in circulating P (n = 4) or those with lower P increases (AC; n = 4). The cells were isolated by Ficoll gradient centrifugation and then cultured in 24-well culture plates using a modified media 199 containing 100 mIU/ml of hMG, FSH, LH, hCG, or a nonhormonal control to stimulate steroid-hormone production. At time points 2, 4, 6, and 8 days, media from each well was frozen for later hormone assay and replaced with fresh media containing the same stimulating factor. After all the media had been collected, P and estradiol (E2) released into the media were measured using radioimmunoassay.

Results

Results indicate a higher media concentration of P(P<0.001), but not E2 (P = 0.254), from NC, regardless of hormone stimulation or time in culture, when compared to the media from AC. Media concentrations of E2 were affected by a cell source by hormone stimulation by time interaction (P<0.001) with varying effects. Media from NC maintained a constant E2 of between 1000–3000 pg/ml over the 8-day period (P=0.163). However, media from AC demonstrated a stimuli-dependent E2 release (P <0.001) ranging from < 1000 to over 11,000 pg/ml.

Conclusions

These data appear to support the existence of functionally different populations of luteinized-granulosa cells from patients undergoing IVF-ET.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. The American Fertility Society, Society for Assisted Reproductive Technology: Assisted reproductive technology in the United States and Canada: 1992 results generated from The American Fertility Society/Society for Assisted Reproductive Technology Registry. Fertil Steril 1994;62:1121–1128

    Google Scholar 

  2. Nayudu PL, Gook DA, Hepworth G, Lopata A, Johnston WIH: Prediction of outcome in human in vitro fertilization based on follicular and stimulation response variables. Fertil Steril 1989;51:117–125

    PubMed  Google Scholar 

  3. Oehninger S, Coddington CC, Scott R, Franken DA, Burkman LJ, Acosta AA, Hodgen GD: Hemizona assay: assessment of sperm dysfunction and prediction of in vitro fertilization outcome. Fertil Steril 1989;51:665–670

    PubMed  Google Scholar 

  4. Bouckaert A, Psalti I, Loumaye E, De Cooman S, Thomas K: The probability of a successful treatment of infertility by in-vitro fertilization. Hum Reprod 1994;9:448–455

    PubMed  Google Scholar 

  5. Stovall DW, Guzick DS, Berga SL, Krasnow JS, Zeleznik AJ: Sperm recovery and survival: two tests that predict in vitro fertilization outcome. Fertil Steril 1994;62:1244–1249

    PubMed  Google Scholar 

  6. Wramsby H, Kullander S, Liedolm P, Rannevik G, Sundstrom P, Thorell J: The success rate of in vitro fertilization of human oocytes in relation to the concentration of different hormones in follicular fluid and peripheral plasma. Fertil Steril 1981;36:448–454

    PubMed  Google Scholar 

  7. Laufer N, DeCherney A, Taratzis B, Naftolin F: The association between preovulatory serum 17B-estradiol patterns and conception in human menopausal gonadotropin-human chorionic gonadotropin stimulation. Fertil Steril 1986;46:73–76

    PubMed  Google Scholar 

  8. Muasher SJ, Oehninger S, Simonetti S, Matta J, Ellis LM, Liu HC, Jones GS, Rosenwaks Z: The value of basal and/or stimulated serum gonadotropin levels in prediction of stimulation response and in vitro fertilization outcome. Fertil Steril 1988;50:298–307

    PubMed  Google Scholar 

  9. Hughes EG, Robertson DM, Handelsman DJ, Hayward S, Healy DL, De Kretser DM: Inhibin and estradiol responses to ovarian hyperstimulation: effects of age and predictive value for in vitro fertilization outcome. J Clin Endocrinol Metab 1990;70:358–364

    PubMed  Google Scholar 

  10. Silverburg KM, Burns WN, Olive DL, Reihl RM, Schenken RS: Serum progesterone levels predict success of in vitro fertilization/embryo transfer in patients stimulated with leuprolide acetate and human menopausal gonadotropins. J Clin Endocrinol Metab 1991;73:797–803

    PubMed  Google Scholar 

  11. Schoolcraft W, Sinton E, Schlenker T, Huynh D, Hamilton F, Meldrum D: Lower pregnancy rate with premature luteinization during pituitary suppression with leuprolide acetate. Fertil Steril 1991;55:563–566

    PubMed  Google Scholar 

  12. Fanchin R, De Fiegler D, Taieb J, Hazout A, Frydman R: Premature elevation of plasma progesterone alters pregnancy rates of in vitro fertilization and embryo transfer. Fertil Steril 1993;59:1090–1094

    PubMed  Google Scholar 

  13. Check JH, Lurie D, Askari HA, Hoover L, Lauer C: The range of subtle rise in serum progesterone levels following controlled ovarian hyperstimulation associated with lower in vitro fertilization pregnancy rates is determined by the source of manufacturer. Eur J Obstet Gynecol Reprod Biol 1993;52:205–209

    PubMed  Google Scholar 

  14. Edelstein MC, Seltman HJ, Cox BJ, Robinson SM, Shaw RA, Muasher SJ: Progesterone levels on the day of human chorionic gonadotropin administration in cycles with gonadotropin-releasing hormone agonist suppression are not predictive of pregnancy outcome. Fertil Steril 1990;54:853–857

    PubMed  Google Scholar 

  15. Hassiakos D, Toner JP, Muasher SJ, Jones HW: Implantation and pregnancy rates in relation to oestradiol and progesterone profiles in cycles with and without the use of gonadotrophin-releasing hormone agonist suppression. Hum Reprod 1990;5:1004–1008

    PubMed  Google Scholar 

  16. Givens CR, Schriock ED, Danderkar PV, Martin MC: Elevated serum progesterone levels on the day of human chorionic gonadotropin administration do not predict outcome in assisted reproduction cycles. Fertil Steril 1994;62:1011–1017

    PubMed  Google Scholar 

  17. Hsiung CH, Karow WG, Gentry WC, Pope AK, Lee TT: Tripling of serum progesterone (P4) and embryo morphological features in conception and nonconception cycles, after human in vitro fertilization. J In Vitro Fertil Embryo Transfer 1988;5:161–166

    Google Scholar 

  18. Prien SD, Canez MS, Messer RH: Increases in progesterone after human chorionic gonadotropin administration may predict cycle outcome in patients undergoing in vitro fertilization-embryo transfer. Fertil Steril 1994;62:1066–1068

    PubMed  Google Scholar 

  19. Tapanainen J, Leinonen PJ, Tapanainen P, Yamamoto M, Jaffe RB: Regulation of human granulosa-luteal cell progesterone and proliferation by gonadotrophins and growth factors. Fertil Steril 0000;48:576–580

  20. Dain LB, Stein P, Krimer AR, Asch RH, De Fried EP, Charreau EH, Tesone M: Progesterone production in cultured human granulosa cells: correlation with follicular fluid hormone levels. Fertil Steril 1991;55:1093–1098

    PubMed  Google Scholar 

  21. Richardson MC, Davies DW, Watson RH, Dunsford ML, Inman CB, Masson GM. Cultured human granulosa cells as a model for corpus luteum function: relative roles of gonadotrophin and low density lipoprotein studied under defined culture conditions. Hum Reprod 1992;7:12–18

    PubMed  Google Scholar 

  22. Jalkanen J: Interaction of 17B-estradiol and progesterone production in human granulosa-luteal cells. Eur J Obstet Gynecol Reprod Biol 1990;37:143–153

    PubMed  Google Scholar 

  23. Bar-Ami S, Khoury C, Zlotkin E, Brandes JM: Increasing progesterone secretion in human granulosa-luteal cells induced by human follicular fluid. Hum Reprod 1993;8:46–52

    PubMed  Google Scholar 

  24. Serta RT, Seibel MM: The influence of epidermal growth factor on progesterone production by human granulosaluteal cells in culture. Hum Reprod 1993;8:1005–1010

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department Ob/Gyn, TTUHSC, Texas Tech University Health Sciences Center, 79430, Lubbock, Texas

    S. D. Prien, M. S. Canez & R. H. Messer

Authors
  1. S. D. Prien
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. S. Canez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. H. Messer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prien, S.D., Canez, M.S. & Messer, R.H. Hormone release from cultured luteinized-granulosa cells mimics differences seenin vivo in patients undergoing IVF-ET. J Assist Reprod Genet 12, 180–186 (1995). https://doi.org/10.1007/BF02211795

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02211795

Key words

Search

Navigation