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Lack of significant effects of superoxide dismutase and catalase on development of reperfusion arrhythmias

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Summary

It has been reported that agents having the ability to scavenge oxygen-derived free radicals reduce the severity of ventricular arrhythmias that occur after brief coronary occlusion and reperfusion. Superoxide dismutase plus catalase (SOD + CAT) or placebo was administered in a blinded randomized fashion prior to coronary occlusion in rats (n = 25 each group) undergoing a 5-min left coronary occlusion followed by 15 min of reperfusion. During reperfusion, ventricular tachycardia (VT) developed in 96 % of animals in both groups. Reperfusion ventricular fibrillation (VF) developed in 60 % of the placebo group vs 56 % in the SOD + CAT group (p =1.0). Irreversible VF occurred in 40 of the placebo group vs 20 % in the SOD + CAT group (p = 0.22). Atrioventricular block occurred in 12 % of placebo and 4 % of SOD + CAT animals (p = 0.61).

There were no significant differences between groups in duration of VT (85 ± 15 s (mean ± SEM) placebo vs 81 ± 14 s SOD + CAT, p = 0.81), total duration of VT plus VF (391 ± 76 s placebo vs 256 ± 64 SOD + CAT, p = 0.45) or numbers of single ventricular ectopic beats (65 ± 15 placebo vs 97 ± 18 SOD + CAT, p = 0.18). Heart rate at reperfusion was slightly higher in control than SOD + CAT animals (340 ± 33 vs 319 ± 32, p = 0.02). Risk zone size, determined by Monastral blue injection, was equal in both groups (34 ± 2 % of ventricular mass). The occurrence of reperfusion VF in this model could not be predicted by heart rate at reperfusion (331 ± 33 VF animals vs 328 ± 36 no VF, p = 0.77), or by risk zone size (34 ± 2 %, VF and no VF groups).

Treatment with SOD + CAT did not reduce the overall incidence or duration of reperfusion arrhythmias in this model, though such treatment was associated with a non-significant trend toward less irreversibility of VF.

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Authors and Affiliations

  1. Hospital of the Good Samaritan, The Heart Institute, 616 S. Witmer Ave, 90017, Los Angeles, California, USA

    J. M. Hagar, S. L. Hale, J. P. Ilvento & R. A. Kloner M.D. Ph.D.

  2. Section of Cardiology, Los Angeles County + University of Southern California Medical Center, Los Angeles, California, USA

    J. M. Hagar, S. L. Hale, J. P. Ilvento & R. A. Kloner M.D. Ph.D.

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  1. J. M. Hagar
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  2. S. L. Hale
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  3. J. P. Ilvento
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  4. R. A. Kloner M.D. Ph.D.
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Supported in part by National Institutes of Health SCOR HL-26215, Bethesda, Maryland 660

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Hagar, J.M., Hale, S.L., Ilvento, J.P. et al. Lack of significant effects of superoxide dismutase and catalase on development of reperfusion arrhythmias. Basic Res Cardiol 86, 127–135 (1991). https://doi.org/10.1007/BF02190545

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  • DOI: https://doi.org/10.1007/BF02190545

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