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Radioimmunoanalysis of antibodies obtained by a liposomal adjuvant

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Abstract

An experimental model for preparing and testing by the radioimmunoanalysis (RIA) method the immunoadjuvant capacity of the dehydration-rehydration vesicles (DRV) over the Freund complete adjuvant (FCA) is presented. The humoral antibody response to bovine serum albumin (BSA), human transferrin and human alphafoetoprotein was investigated on rats. The concentration of specific antibodies (a) and the average antibody affinity, constant (k) are the two intrinsic parameters of the antisera measured by RIA we referred to. The experimental results show thatk is higher anda is smaller for the liposomal adjuvant than for the Freund one.

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Authors and Affiliations

  1. Institute of Atomic Physics, IFIN, Lab. 8, P.O.Box MG-6, R-76900, Magurele-Bucharest, Romania

    Anca Bejan & I. Dorobantu

  2. Faculty of Biology, Splaiul Independentei 91-95, R-76201, Bucharest, Romania

    Maria Suciu

  3. “Dr. I. Cantacuzino” Institute, Splaiul Independentei 103, R-70100, Bucharest, Romania

    Anca Maican

  4. Faculty of Physics, Biophysics Department, P.O.Box Mg-6, R-76900, Magurele-Bucharest, Romania

    Gr. Turcu

Authors
  1. Anca Bejan
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  2. Maria Suciu
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  3. I. Dorobantu
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  4. Anca Maican
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  5. Gr. Turcu
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Bejan, A., Suciu, M., Dorobantu, I. et al. Radioimmunoanalysis of antibodies obtained by a liposomal adjuvant. Journal of Radioanalytical and Nuclear Chemistry Letters 200, 475–482 (1995). https://doi.org/10.1007/BF02162496

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  • DOI: https://doi.org/10.1007/BF02162496

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