Journal of Cancer Research and Clinical Oncology

, Volume 114, Issue 4, pp 373–379 | Cite as

Analysis of metastatic competence of mouse bladder carcinoma cells after transfection with activated Ha-ras or N-ras oncogenes

  • J. Pohl
  • A. Radler-Pohl
  • L. M. Franks
  • V. Schirrmacher
Original Papers Experimental Oncology


Transfection of the Ha-ras oncogene into a low metastatic epithelial cell line resulted in the acquirement of significantly increased metastatic capacity. This alteration in metastatic competence of a carcinoma line in a syngeneic system seemed to be a selective change and was not affected by parameters such as tumor latency period or local tumor growth. Transfection of the selection marker vectors with normal cellular DNA or with the N-ras gene did not lead to significantly increased metastatic capacity. Analysis of metastatic variants after oncogene transfection and in vivo selection showed integration of N-ras, but not of Ha-ras oncogenes. A possible role for the Ha-ras oncogene in the initial steps of metastasis will be discussed.

Key words

Oncogene Ras Metastasis Transfection 


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • J. Pohl
    • 1
  • A. Radler-Pohl
    • 1
  • L. M. Franks
    • 2
  • V. Schirrmacher
    • 1
  1. 1.German Cancer Research CenterInstitute for Immunology and GeneticsHeidelbergGermany
  2. 2.Department of Cellular PathologyImperial Cancer ResearchLincoln's Inn FieldUK

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