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Zur Isolierung und qualitativen Bausteinanalyse der K-Antigene von Klebsiellen

Isolation and qualitative component analysis of Klebsiella K-antigens

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Summary

The capsular antigens of Klebsiella types 1–72 were obtained by extraction with phenol/water followed by cetavlon purification. The K antigens were found to be acidic heteropolysaccharides consisting of seven different monosaccharide constituents when analysed by comparative paper chromatography. Mostly glucuronic acid represents the acidic component. Galacturonic acid was found to be present only in six antigens. The capsular polysaccharides contain at least two other sugars in addition. Various combinations of monosaccharides were observed. Some of these have the same qualitative sugar composition but differ with respect to their serological specificity.

Zusammenfassung

Die Kapselantigene der Klebsiella-Typen K1–K72 wurden mit Phenol/Wasser extrahiert und mit Hilfe von Cetavlon gereinigt. Die qualitative Bausteinanalyse hat ergeben, daß die K-Antigene der Klebsiellen saure Heteropolysaccharide sind, an deren Aufbau sieben verschiedene Monosaccharide beteiligt sind. In den meisten Fällen stellt Glucuronsäure die saure Komponente dar. Galacturonsäure tritt viel seltener auf. Außer einer Hexuronsäure enthalten die K-Antigene mindestens zwei weitere Bausteine (Hexosen, 6-Desoxyhexosen). Es wurden verschiedene Zuckerbaustein-Kombinationen beobachtet. Einige kommen sogar mehrfach vor, wobei die qualitativ gleiche Zusammensetzung der K-Antigene nicht mit serologischer Identität einhergeht.

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  1. Mikrobiologisch-Immunologische Abteilung des Instituts für Medizinische Mikrobiologie und Epidemiologie der Universität Rostock, Deutschland

    Wolfgang Nimmich

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Der techn. Assistentin, Fr. Britta Becziczka, sind wir für zuverlässige Mitarbeit sehr zu Dank verpflichtet.

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Nimmich, W. Zur Isolierung und qualitativen Bausteinanalyse der K-Antigene von Klebsiellen. Z. med. Mikrobiol. u. Immunol. 154, 117–131 (1968). https://doi.org/10.1007/BF02123700

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