Abstract
The extracellular soluble proteins of 15 different isolates comprising 9 different physiological races ofCladosporium fulvum were harvested from 3 to 4 weeks old shake cultures. The culture filtrates were purified by gel filtration over Sephadex G-25 and the Vo-fractions were freeze-dried. These preparations were submitted to disc gel electrophoresis on polyacrylamide gels at pH 9.5 at circa 4°C.
The electrophoretical protein patterns appeared to differ for each isolate irrespective of the physiological race to which they belong. The various isolates originated from different sites in the Netherlands.
Samenvatting
De extracellulaire oplosbare eiwitten van 15 verschillende isolaten, behorend tot 9 verschillende fysiologische rassen vanCladosporium fulvum, werden verkregen met behulp van Sephadex G-25 gelfiltratie van 3 tot 4-weken-oude filtraten van schudcultures. De drooggevroren fracties werden onderworpen aan elektroforese op polyacryl amide gels bij pH 9.5 en ongeveer 4°C.
De met elektroforese verkregen eiwitpatronen bleken verschillend voor ieder isolaat onafhankelijk van het fysio waartoe zij behoren. Deze isolaten waren van verschillende plaatsen in Nederland afkomstig.
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Van Dijkman, A., Dieleman, S.J. & Sijpesteijn, A.K. Differences in disc gel electrophorese pattern of soluble proteins excreted by different physiological races and isolates of Cladosporium fulvum. Netherlands Journal of Plant Pathology 79, 70–80 (1973). https://doi.org/10.1007/BF02083669
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DOI: https://doi.org/10.1007/BF02083669