Abstract
Three auxin-inducible genes of the soybean SAUR (small auxin up RNAs) locus, along with two flanking genes, have been transferred into petunia usingAgrobacterium tumefaciens T-DNA transformation. Genomic analysis showed that two transgenic petunia plants contained one copy of the SAUR locus, and one plant contained approximately 10 copies of the transferred locus. Gene-specific probes were used to probe RNA blots, and our results indicated that the three genes were transcriptionally induced by the addition of the auxin, 2,4-dichlorophenoxyacetic acid (2,4-D) to excised young leaves of the transgenic plants. This suggests that elements involved in auxin-regulated gene expression are strongly conserved in soybean and petunia. The two plants containing one copy of the SAUR locus showed nearly equal expression of all three SAUR genes after 2,4-D induction, while the plant with 10 copies showed a dramatic under-expression of two of the three genes following auxin administration. RNase protection analysis of the transcript for SAUR locus gene 6B showed that the transcription start site for this gene in the three transformed petunia plants was identical to that in soybean.
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Paper No. 10809 of the Journal Series of the Missouri Agricultural Experiment Station.
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Wright, R., McClure, B. & Guilfoyle, T. Auxin-induced expression of the soybean SAUR locus in transgenic petunia. J Plant Growth Regul 8, 283–291 (1989). https://doi.org/10.1007/BF02021821
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DOI: https://doi.org/10.1007/BF02021821