Skip to main content
SpringerLink
Log in

Characterization of a class of small auxin-inducible soybean polyadenylated RNAs

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Four new auxin-responsive RNAs from soybean (Glycine max (L.) Merr., var. Wayne) are described. The RNAs were identified by hybridization to three cDNA probes obtained from a library enriched for sequences which increase in abundance within 60 min after 2,4-D (2,4-dichlorophenoxyacetic acid) treatment. These RNAs appear to define a new class of small (i.e. approximately 550 nucleotides) RNAs that respond extremely rapidly to application of exogenous auxin. In excised elongating hypocotyl sections, an increase in the abundance of these RNAs can be detected 2 to 5 min after treatment with 50 μM 2,4-D. This response is half maximal after 10 min and reaches steady state in 60 min. RNA blot analysis shows that these RNAs are expressed differentially in various parts of the seedling. The degree of inducibility by auxin is also organ-specific, with the elongating hypocotyl being the most responsive of the organs tested. The RNAs display identical response specificities with one exception. Accumulation of one RNA, designated 10A, is completely abolished by simultaneous addition of cycloheximide and 2,4-D. This RNA also displays a different 2,4-D dose response than other RNAs examined. These results suggest that more than one mechanism is involved in rapid modulation of gene expression by auxin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bantle JA, Maxwell IA, Hahn WE: Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA. Anal Biochem 72: 413–427 (1976).

    Google Scholar 

  2. Baulcombe DC, Key JL: Polyadenylated RNA sequences which are reduced in concentration following auxin treatment of soybean hypocotyl. J Biol Chem 255: 8907–8913 (1980).

    Google Scholar 

  3. Berger SL, Birkenmeir CS: Inhibition of intractable nucleases with ribonucleoside-vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes. Biochemistry 18: 5143–5149 (1979).

    Google Scholar 

  4. Czarnecka EL, Edelman L, Schoffl F, Key JL: Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs. Plant Mol Biol 3: 45–58 (1984).

    Google Scholar 

  5. Davis MM, Cohen DI, Neilsen EA, Steinmetz M, Paul WE, Hood L: Cell-type-specific cDNA probes and the murine I region: The localization and orientation of Aα d. Proc Natl Acad Sci USA 81: 2194–2198 (1984).

    Google Scholar 

  6. Dente L, Cesareni G, Cortese R: pEMBL: a new family of single stranded plasmids. Nucl Acids Res 11: 1645–1655 (1983).

    Google Scholar 

  7. Evans ML, Ray PM: Timing of the auxin response in coleoptiles and its implications regarding auxin action. J Gen Physiol 53: 1–20 (1969).

    Google Scholar 

  8. Glisin V, Crkvenjakov R, Byrus C: Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13: 2633–2637 (1974).

    Google Scholar 

  9. Guilfoyle TJ: Auxin-regulated gene expression in higher plants. CRC Crit Rev Plant Sci 4: 247–276 (1986).

    Google Scholar 

  10. Hagen G, Kleinschmidt A, Guilfoyle T: Auxin-regulated gene expression in intact soybean hypocotyl and excised hypocotyl sections. Planta 162: 147–153 (1984).

    Google Scholar 

  11. Hagen G, Guilfoyle TJ: Rapid induction of selective transcription by auxins. Mol Cell Biol 5: 1197–1203 (1985).

    Google Scholar 

  12. Hu N, Messing J: The making of strand-specific M13 probes. Gene 17: 271–277 (1982).

    Google Scholar 

  13. Huynh TV, Young RA, Davis RW: Constructing and screening cDNA libraries in λ-gt10 and λ-gt11. In: Glover DM (ed.) DNA Cloning Techniques: A Practical Approach, Vol. 1. IRL Press, London (1985) pp 49–78.

    Google Scholar 

  14. Jones JF, Kende H: Auxin-included ethylene biosynthesis in subapical stem sections of etiolated seedlings of Pisum sativum L.. Planta 146: 649–656 (1979).

    Google Scholar 

  15. Lizardi PM, Engelberg A: Rapid isolation of RNA using proteinase K and sodium perchlorate. Anal Biochem 98: 116–122 (1979).

    Google Scholar 

  16. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1982) pp 204.

    Google Scholar 

  17. Marre E: Fusicoccin: a tool in plant physiology. Ann Rev Plant Physiol 30: 273–288 (1979).

    Google Scholar 

  18. McMaster GK, Carmichael GG: Analysis of Single and double-stranded nucleic acid on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci USA 74: 4835–4838 (1977).

    Google Scholar 

  19. Mozer TJ: Partial purification and characterization of the mRNA for α-amylase from barley aleurone layers. Plant Physiol 65: 834–837 (1980).

    Google Scholar 

  20. Okayama H, Berg P: High-efficiency cloning of full-length cDNA. Mol Cell Biol 2: 161–170 (1982).

    Google Scholar 

  21. Theologis A, Ray PM: Early auxin-regulated polyadenylated mRNA sequences in pea stem tissue. Proc Natl Acad Sci USA 79: 418–421 (1982).

    Google Scholar 

  22. Theologis A, Huynh TV, Davis RW: Rapid induction of specific mRNAs by auxin in pea epicotyl tissue. J Mol Biol 183: 53–68 (1985).

    Google Scholar 

  23. Thimann K: The synthetic auxins. Relationship between structure and activity. In: Skoog F (ed.) Plant Growth Substances University of Wisconsin Press, Madison (1951) pp 21–36.

    Google Scholar 

  24. Vanderhoef LN, Stahl CA, Siegel N, Zeigler R: The inhibition by cytokinin of auxin-promoted elongation in excised soybean hypocoty. Physiol Plant 29: 22–27 (1973).

    Google Scholar 

  25. Vanderhoef LN, Stahl CA, Lu T-YS: Two elongation responses to auxin respond differently to protein synthesis inhibition. Plant Physiol 58: 402–404 (1976).

    Google Scholar 

  26. Vanderhoef LN: In: Skoog F (ed.) Plant Growth Substances 1979. Springer-Verlag, Heidelberg (1980) pp 90–96.

    Google Scholar 

  27. Walker JC, Key JL: Isolation of clone cDNAs to auxin-reponsive poly(A)+ RNAs of elongating soybean hypocotyl. Proc Natl Acad Sci USA 79: 7185–7189 (1982).

    Google Scholar 

  28. Walker JC, Legocka J, Edelman L, Key JL: An analysis of growth regulator interactions and gene expression during auxin-induced cell elongation using cloned complementary DNAs to auxin-responsive messenger RNAs. Plant Physiol 77: 847–850 (1985).

    Google Scholar 

  29. Zurfluh LL, Guilfoyle TJ: Auxin-induced changes in the population of translatable messenger RNA in elongating sections of soybean hypocotyl. Plant Physiol 69: 332–337 (1982).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Minnesota, 55108, St. Paul, MN, USA

    Bruce A. McClure

  2. Department of Biochemistry, University of Missouri, 65211, Columbia, MO, USA

    Tom Guilfoyle

Authors
  1. Bruce A. McClure
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tom Guilfoyle
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

McClure, B.A., Guilfoyle, T. Characterization of a class of small auxin-inducible soybean polyadenylated RNAs. Plant Mol Biol 9, 611–623 (1987). https://doi.org/10.1007/BF00020537

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00020537

Key words

Search

Navigation