Abstract
Monoclonal antibodies were produced againstEscherichia coli O111, Escherichia coli J5, and the rough (R) mutant ofSalmonella typhimurium M206, and tested by enzyme-linked immunosorbent assay against lipopolysaccharides of several gram-negative strains. The monoclonal antibodies were also identified with an immunoblotting assay.Anti-Escherichia coli O111 monoclonal antibodies reacted only with homologous O antigens. Anti-J5 monoclonal antibodies cross-reacted with core lipopolysaccharide, especially with Rc lipopolysaccharide. IgM anti-J5 monoclonal antibodies showed more extensive cross-reactivity than IgG3 monoclonal antibodies. Anti-Re monoclonal antibodies cross-reacted weakly with all rough lipopolysaccharide tested. Thus, the varying specificity of these monoclonal antibodies seems to indicate that the core regions in the lipopolysaccharides of various gramnegative bacteria are not similar.
Similar content being viewed by others
References
Westphal, O.: Bacterial endotoxins. International Archives of Allergy and Applied Immunology 1975, 49: 1–43.
Schellekens, J. F. P., Kalter, E. S., Vreede, R. W., Verhoef, J.: Host-parasite interaction in serious infections due to gram-negative bacteria. Antonie van Leeuwenhoek 1984, 50: 701–710.
Ziegler, E. J., MacCutchan, J. A., Fiere, J., Glauser, M. P., Sadoff, J. C., Douglas, H., Braude, A. I.: Treatment of gram-negative bacteremia and shock with human antiserum to a mutantEscherichia coli. New England Journal of Medicine 1982, 307: 1225–1230.
Elbein, A. D., Jeath, E. C.: The biosynthesis of cell wall lipopolysaccharide inEscherichia coli. I. The biochemical properties of a uridine diphosphate galactose 4-epimeraseless mutant. Journal of Biological Chemistry 1975, 240: 1919–1925.
Lüderitz, O., Staub, A. M., Westphal, O.: Immunochemistry of O and R antigensof Salmonella and relatedEnterobacteriaceae. Bacteriological Review 1966, 30: 192–255.
Braude, A. I., Ziegler, E. J., Douglas, H., MacCutchan, J. A.: Antibody to cell wall glycolipid of gram-negative bacteria: Induction of immunity to bacteremia and endotoxemia. Journal of Infectious Diseases 1977, 126: S167-S173.
Baumgartner, J. D., McCutchan, J. A. van Melle, G., Vogt, M., Luethy, R., Glauser, M. P., Ziegler, E. J., Klauber, M. R., Muehlen, E., Chiolero, R., Geroulanos, S.: Prevention of gram-negative shock and death in surgical patients by antibody to endotoxin core glycolipid. Lancet 1985, ii: 59–60.
Kirkland, T. N., Ziegler, E. J.: An immunoprotective monoclonal antibody to lipopolysaccharide. Journal of Immunology 1984, 132: 1330–2590.
Nelles, M. J., Niswander, C. A.: Mouse monoclonal antibodies reactive with J5 lipopolysaccharide exhibit extensive serological cross-reactivity with a variety of gram-negative bacteria. Infections and Immunity 1984, 46: 677–681.
Young, L. S.: Monoclonal antibodies: technology and application to gram-negative infections. Infection 1985, 13, Supplement 2: 224–226.
Galanos, C., Lüderitz, O., Rietschel, E. T., Westphal, O.: Biochemistry of lipids II. International Review of Biochemistry 1977, 14: 290–292.
Shulman, M., Wilde, C. D., Köhler, G.: A better cell line for making hybridomas secreting specific antibodies. Nature 1978, 276: 269–270.
Köhler, G., Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975, 256: 495–497.
Fazekas de St. Groth, S. Scheidegger, D.: Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 1980, 35: 1–21.
Lugtenberg, B., Meyers, J., Peters, R., van der Hoek, P., van Alphen, L.: Electrophoretic resolution of the major outer membrane protein ofEscherichia coli K12 into four bands. FEBS Letters 1985, 58: 254–258.
Tsai, C. M., Fragsch, C. E.: A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 1982, 119: 115–119.
Burnette, W. N.: “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytic Biochemistry 1981, 112: 195–203.
Hunt, F.: Pattern of LPS synthesis in gram-negative bacteria. Journal of Theoretic Biology 1985, 115: 213–219.
de Jongh-Leuvenink, J., Vreede, R. W., Marcelis, J. H., de Vos, M., Verhoef, J.: Detection of antibodies against lipopolysaccharides ofEscherichia coli andSalmonella R and S strains by immunoblotting. Infection and Immunity 1985, 50: 716–720.
Brade, L., Brade, H.: Characterization of two different antibody specificities recognizing distinct antigenic determinants in free lipid A ofEscherichia coli. Infection and Immunity 1985, 48: 776–781.
Teng, N. N. H., Kaplan, H. S., Hebert, J. M., Moore, C., Douglas, H., Wunderlich, A., Braude, A. I.: Protection against gram-negative bacteremia and endotoxemia with human monoclonal IgM antibodies. Proceedings of the National Academy of Science of the United States of America 1985, 82: 1790–1794.
Van Alphen, W., Lugtenberg, B., Berendsen, W.: Heptosedeficient mutants ofEscherichia coli K12 deficient in up to three major outer membrane proteins. Molecular General Genetics 1976, 147: 263–269.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Jongh-Leuvenink, J., Bouter, A.S., Marcelis, J.H. et al. Cross-reactivity of monoclonal antibodies against lipopolysaccharides of gram-negative bacteria. Eur. J, Clin. Microbiol. 5, 148–151 (1986). https://doi.org/10.1007/BF02013970
Issue Date:
DOI: https://doi.org/10.1007/BF02013970