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The interaction of collagenase with hydroxyapatite and related materials and enzymatic properties of the adsorbed enzyme

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Summary

The commerical collagenase fromClostridium histolyticum was adsorbed on hydroxyapatite, on bovine femur shaft and on enamel and dentin powders. The substrate specificity of the adsorbed enzyme tested, with chromophore substrate, azocoll and native collagen, differed from that obtained with the soluble enzyme. The adsorption and the substrate specificity was also dependent on the adsorbent used. A pretreatment of hydroxyapatite with chondroitin sulphate, hyaluronate and DNA lowered the adsorption of collagenase. Phosphate ion caused desorption of the enzyme from hydroxyapatite. Sodium fluoride caused partial desorption of the enzyme from hydroxyapatite and enamel and dentin powders. Collagenase adsorbed on root surfaces of teeth liberated hydroxyproline containing material.

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Author notes

  1. M. L. E. Knuuttila

    Present address: Department of Periodontology, Institute of Dentistry, University of Kuopio, Pl 138, Kuopio 10, Finland

Authors and Affiliations

  1. Department of Periodontology, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, 20520, Turku 52, Finland

    M. L. E. Knuuttila & K. U. Paunio

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  1. M. L. E. Knuuttila
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  2. K. U. Paunio
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Knuuttila, M.L.E., Paunio, K.U. The interaction of collagenase with hydroxyapatite and related materials and enzymatic properties of the adsorbed enzyme. Calc. Tis Res. 25, 127–131 (1978). https://doi.org/10.1007/BF02010761

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  • DOI: https://doi.org/10.1007/BF02010761

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