Summary
For obtaining a better yield of established lines of embryonicDrosophila cells, insulin proved to be a useful substance to be added to the culture medium. 10% of lines became established, showing a predominatly diploid chromosome number.
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Mosna, G., Barigozzi, C. Stimulation of growth by insulin inDrosophila embryonic cells in vitro. Experientia 32, 855–856 (1976). https://doi.org/10.1007/BF02003727
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DOI: https://doi.org/10.1007/BF02003727