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In vitrol culture ofDrosophila melanogaster embryonic cells

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Summary

A new culture medium for cells fromDrosophila embryos has been designed. It has an osmotic pressure equivalent to 10.5 g per liter of NaCl (pH 6.7), Na+: K+ ratio of 1.4, and very low Cl−. Glycine and glutamic acid are high. Cells grow well for several weeks in this medium at 25–27°C and some cultures persist for months and establish euploid lines.

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References

  1. Grace, T. D. C. 1966. Establishment of a line of mosquito (Aedes aegypti L.) cells grownin vitro. Nature (London) 211: 366–367.

    Article  CAS  Google Scholar 

  2. Singh, K. R. P. 1967. Cell cultures derived from larvae ofAedes albopictus (Skuse) andAedes aegypti (L.). Curr. Sci. 36: 506–508.

    Google Scholar 

  3. Peleg, J. 1968. Growth of arboviruses in monolayers from subcultured mosquito embryo cells. Virology 35: 617–619.

    Article  PubMed  CAS  Google Scholar 

  4. Schneider, I. 1969. Establishment of 3 diploid cell lines ofAnopheles stephensi (Diptera:Culicidae). J. Cell. Biol. 42: 603–606.

    Article  PubMed  CAS  Google Scholar 

  5. Barigozzi, C., S. Dolfini, M. Fraccaro, G. Rezzonico-Raimondi, and L. Tiepolo. 1966.In vitro study of the DNA replication patterns of somatic chromosomes ofDrosophila melanogaster. Exp. Cell. Res. 43: 231–234.

    Article  PubMed  CAS  Google Scholar 

  6. Castiglioni, M. C., and G. Rezzonico-Raimondi. 1961. First results of tissue culture inDrosophila. Experientia 17: 88–90.

    Article  PubMed  CAS  Google Scholar 

  7. Castiglioni, M. C., and G. Rezzonico-Raimondi. 1963. Genotypical differences between stocks ofDrosophila melanogaster revealed from culturingin vitro. Experientia 19: 527–529.

    Article  PubMed  CAS  Google Scholar 

  8. Dolfini, S., and A. Gottardi. 1966. Changes of chromsome number in cells ofDrosophila melanogaster culturedin vitro. Experientia 22: 144.

    Article  PubMed  CAS  Google Scholar 

  9. Fox, A. S., M. Horikawa, and L. N. L. Ling. 1968. The use ofDrosophila cell cultures in studies of differentiation. In Vitro 3: 65–84.

    Google Scholar 

  10. Horikawa, M. 1958. Developmental genetic studies of tissue cultured eye discs ofDrosophila melanogaster. I. Growth, differentiation and tryptophan metabolism. Cytologia 23: 468–477.

    Google Scholar 

  11. Horikawa, M., and A. S. Fox. 1964. Culture of embryonic cells ofDrosophila melanogaster in vitro. Science 145: 1437–1439.

    Article  PubMed  CAS  Google Scholar 

  12. Horikawa, M., L. N. L. Ling, and A. S. Fox. 1966. Long-term culture of embryonic cells ofDrosophila melanogaster. Nature (London) 210: 183–185.

    Article  CAS  Google Scholar 

  13. Gvosdev, V. A., and V. T. Kakpakov. 1968. Culture of embryonic cells ofDrosophila melanogaster in vitro (in Russian). Genetika 4: 129–142.

    Google Scholar 

  14. Kuroda, Y. 1954. The culture of the eye-discs ofDrosophila. Zool. Mag. 63: 75.

    Google Scholar 

  15. Kuroda, Y. 1954. The culture of wing-discs ofDrosophila melanogaster. Jap. J. Genet. 29: 163.

    Google Scholar 

  16. Kuroda, Y. 1969. Characteristic aggregation pattern of dissociated imaginal discs cells ofDrosophila melanogaster larvae in rotation culture. Drosophila Inf. Serv. 44: 109–110.

    Google Scholar 

  17. Kuroda, Y., and S. Tamura. 1956. A technic for the tissue culture of melanotic tumors of Drosophila melanogaster in the synthetic medium. Med. J. Osaka Univ. 7: 137–144.

    Google Scholar 

  18. Robb, J. A. 1969. Maintenance of imaginal discs ofDrosophila melanogaster in chemically defined media. J. Cell Biol. 41: 876–885.

    Article  PubMed  CAS  Google Scholar 

  19. Shields, G., and J. H. Sang. 1970. Characteristics of five cell types appearing duringin vitro. culture of embryonic material fromDrosophila melanogaster. J. Embryol. Exp. Morph. 23: 53–69.

    PubMed  CAS  Google Scholar 

  20. Schneider, I. 1963.In vitro culture ofDrosophila organs and tissues. Genetics 48: 908.

    Google Scholar 

  21. Schneider, I. 1964. Differentiation of larvalDrosophila eye-antennal discsin vitro. J. Exp. Zool. 156: 91–104.

    Article  PubMed  CAS  Google Scholar 

  22. Seecof, R. L., and R. L. Unanue. 1968. Differenentiation of embryonicDrosophila cellsin vitro. Exp. Cell. Res. 50: 654–660.

    Article  PubMed  CAS  Google Scholar 

  23. Kakpakov, V. T., V. A. Gvosdey, T. P. Platova, and L. G. Polukarova. 1969.In vitro establishment of embryonic cell lines ofDrosophila melanogaster. Genetika 5: 67–75.

    Google Scholar 

  24. Echalier, G., A. Ohanessian, and G. Brun. 1965. Cultures “primaires” de cellules embryonnaires deDrosophila melanogaster (Insecte Diptère). C. R. Acad. Sci. (Paris) 261: 3211–3213.

    CAS  Google Scholar 

  25. Echalier, G., and A. Ohanessian. 1969. Isolement, en culturesin vitro, de lignées cellulaires diploïdes deDrosophila melanogaster. C. R. Acad. Sci. (Paris) 268: 1771–1773.

    CAS  Google Scholar 

  26. Zwicky, K. 1954. Osmoregulatorische Reaktionen der Larve vonDrosophila melanogaster. Z. Vergl. Physiol. 36: 367.

    Article  Google Scholar 

  27. Begg, M. 1955. Osmotic pressure ofDrosophila larval haemolymph. Drosophila Inf. Serv. 29: 105.

    Google Scholar 

  28. Begg, M., and W. J. Cruickshank. 1963. A partial analysis ofDrosophila larval haemolymph. Proc. Roy. Soc. (Edinburgh), 68: 215–236.

    Google Scholar 

  29. Croghan, P. C., and A. P. M. Lockwood. 1960. The composition of the haemolymph of the larva ofDrosophila melanogaster. J. Exp. Biol. 37: 339–344.

    CAS  Google Scholar 

  30. Gloor, H., and P. S. Chen. 1950. Uber ein Analorgan beiDrosophila Larven. Rev. Suisse Zool. 57: 570–576.

    Google Scholar 

  31. Wyatt, S. S. 1956. Culturein vitro of tissue from the SilkwormBombyx-mori L. J. Gen. Physiol. 39: 841–852.

    Article  PubMed  CAS  Google Scholar 

  32. Shaw, E. I. 1956. A glutamic acid-glycine medium for prolonged maintenance of high mitotic activity in grasshopper neuroblasts. Exp. Cell Res. 11: 580–586.

    Article  PubMed  CAS  Google Scholar 

  33. Grace, T. D. C., and W. H. Brzostowski. 1966. Analysis of the amino acids and sugars in an insect cell culture medium during cell growth. J. Insect Physiol. 12: 625–633.

    Article  CAS  Google Scholar 

  34. Landureau, J. C., and P. Jolles. 1969. Etude des exigences d'une lignée de cellules d'Insectes (source EPa). I. Acides aminés. Exp. Cell Res. 54: 391–398.

    Article  PubMed  CAS  Google Scholar 

  35. von der Crone-Gloor, U. 1959. Quantitative Untersuchung der freien Aminosäuren und Polypeptide während der embryonalentwicklung vonDrosophila melanogaster. J. Insect Physiol. 3: 50–56.

    Article  Google Scholar 

  36. Wyatt, G. R. 1961. The biochemistry of insect hemolymph. Ann. Rey. Entomol. 6: 75–102.

    Article  CAS  Google Scholar 

  37. Grace, T. D. C. 1962. Establishment of four strains of cells from insect tissues grownin vitro. Nature (London) 1951: 788–789.

  38. Wyatt, G. R., and G. F. Kalf. 1957. The chemistry of insect hemolymph II. Trehalose and other carbohydrates. J. Gen. Physiol. 40: 833–847.

    Article  PubMed  CAS  Google Scholar 

  39. Brun, G. 1963. Etude d'une association du virus δ et de son hôte la Drosophile: l'état stabilisé. Thèse, Orsay, 1–255.

  40. Vago, C., and P. Flandre. 1963. Culture prolongée de tissus d'insectes et de vecteurs de maladies en coagulum plasmatique. Ann. Epiphyties 14: 127–139.

    Google Scholar 

  41. Richard-Molard, C. Unpublished data.

  42. Dolfini, S., A. M. Courgeon, and L. Tiepolo. 1970. The cell cycle of an established line ofDrosophila melanogaster cellsin vitro. Experientia. To be published.

  43. Barigozzi, C. 1970.Drosophila cellsin vitro: behaviour and utilization for genetic purpose. In:Symposium on Arthropod Cell Cultures, Bethesda, March 1970. To be published.

  44. Echalier, G. 1970. The dipoloid lines ofDrosophila cells as a material for physiologic genetics of somatic cells: isolation of clones; selection of biochemical mutants. In:Symposium on Arthropod Cell Cultures, Bethesda, March 1970. To be published.

  45. l'Héritier, Ph. 1958. The hereditary virus ofDrosophila. Advance Virus Res. 5: 195–245.

    Article  PubMed  Google Scholar 

  46. Ohanessian, A., and G. Echalier. 1967. Multiplication ofDrosophila hereditary virus (δ virus) inDrosophila embryonic cells cultivatedin vitro. Nature (London) 213: 1049–1050.

    Article  Google Scholar 

  47. Ohanessian, A. 1970. Sigma virus multiplication inDrosophila cell cultures of different geno-types. In:Symposium on Arthropod Cell Cultures, Bethesda, March 1970. To be published.

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Authors and Affiliations

  1. Laboratoire de Biologie Animale (PCEM), Faculté des Sciences, Paris, France

    G. Echalier & A. Ohanessian

  2. Laboratoire de Génétique des Virus, CNRS, 91, Gif sur Yvette, France

    G. Echalier & A. Ohanessian

Authors
  1. G. Echalier
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  2. A. Ohanessian
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Echalier, G., Ohanessian, A. In vitrol culture ofDrosophila melanogaster embryonic cells. In Vitro 6, 162–172 (1970). https://doi.org/10.1007/BF02617759

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