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Macrophage-released neutrophil chemotactic factor (MNCF) induces PMN-neutrophil migration through lectin-like activity

  • Cell Adhesion Molecules in Inflammation
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Abstract

We have previously reported that rat peritoneal macrophages stimulated with LPS release a factor (MNCF) which induces neutrophil migration that is not blocked by glucocorticoids. The supernatant of macrophage monolayers stimulated with LPS was submitted to affinity chromatography on immobilized sugar columns. We observed that thed-gal binding fraction retained MNCF activity. This fraction, consisting of four protein components, was submitted to chromatography on Superdex 75, yielding a homogeneous preparation of the active component. MNCF has a MW of 54 KDa (gel filtration and SDS-PAGE) and pI<4.0 (isoelectrofocusing and chromatofocusing).d-gal did not interfere with the behaviour of known interleukins (IL-1β, IL-6, IL-8 TNF-α), but blocked MNCF activity in anin vitro migration assay. The present results reinforce our previous suggestion that MNCF may correspond to a novel monokine which induces neutrophil migration through a direct mechanism involving thed-gal binding site of the molecule.

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References

  1. S. H. Ferreira,Are macrophages the body's alarm cells? Agents and Action10, 229 (1980).

    Google Scholar 

  2. E. R. Unanue and P. M. Allen,The basis for immunoregulatory role of macrophages and other accessory cells. Science236, 551–557 (1987).

    PubMed  Google Scholar 

  3. C. F. Nathan,Secretory products of macrophages. J. Clin. Invest.79, 319–326 (1987).

    PubMed  Google Scholar 

  4. F. Q. Cunha and S. H. Ferreira,The release of a neutrophils chemotactic factor from peritoneal macrophages by endotoxin: Inhibition by glucocorticoids. Eur. J. Pharmacol.129, 65–76 (1986).

    PubMed  Google Scholar 

  5. G. E. P. Souza, F. Q. Cunha, R. Mello, and S. H. Ferreira,Neutrophil migration induced by inflammatory stimuli is reduced by macrophage depletion. Agents and Actions24, 377–380 (1988).

    PubMed  Google Scholar 

  6. I. G. Colditz,Margination and emigration of leucocytes. Surv. Synth. Path. Res.4, 44–68 (1985).

    Google Scholar 

  7. L. A. Lasky,Lectin Cell Adhesion Molecules (LEC-CAMs): A new family of cell adhesion proteins involved with inflammation. J. Cell Biochem.45, 139–146 (1991).

    PubMed  Google Scholar 

  8. L. A. Lasky,Selectins: Interpreters of cell-specific carbohydrate information during inflammation. Science258, 964–969 (1992).

    PubMed  Google Scholar 

  9. K. A. Karlsson,Glycobiology: A growing field for drug design. TiPS12, 265–272 (1991).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Immunology, Faculty of Medicine, 14.049-900, Ribeirão Preto São, Paulo, Brazil

    M. Dias-Baruffi & M. C. Roque-Barreira

  2. Department of Pharmacology, Faculty of Medicine, 14.049-900, Ribeirão Preto São, Paulo, Brazil

    F. Q. Cunha & S. H. Ferreira

Authors
  1. M. Dias-Baruffi
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  2. F. Q. Cunha
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  3. S. H. Ferreira
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  4. M. C. Roque-Barreira
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Dias-Baruffi, M., Cunha, F.Q., Ferreira, S.H. et al. Macrophage-released neutrophil chemotactic factor (MNCF) induces PMN-neutrophil migration through lectin-like activity. Agents and Actions 38 (Suppl 2), C54–C56 (1993). https://doi.org/10.1007/BF01991135

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  • DOI: https://doi.org/10.1007/BF01991135

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